Abstract: Grouper is a rare seawater fish with delicious taste and rich nutrition. In our previous study, we bred the hybrid “Jinhu grouper” with a growth advantage by crossing the frozen sperm of Epinephelus tukula and E. fuscoguttatus egg. The average weight of 1 age old “Jinhu grouper” was 559.76 g, 1.63 times that of the hybrid of E. fuscoguttatus♀× E. lanceolatus♂. In this study, the compositions and contents of water, protein, crude fat, ash content, amino acids, fatty acids and 10 mineral elements in the muscles of E. fuscoguttatus, E. fuscoguttatus♀× E. tukula♂ andE. fuscoguttatus♀× E. lanceolatus♂ were measured by conventional methods, according to the national standard. The crude protein contents in the muscles of E. fuscoguttatus, E. fuscoguttatus♀× E. tukula♂ and E. fuscoguttatus♀× E. lanceolatus♂ were 19.0%, 20.2% and 16.8% respectively. The total quantity of amino acids, essential amino acids, essential amino acid index and the fresh amino acids of these three groupers were (18.10%, 18.53%, 16.01%), (7.52%, 7.70%, 6.32%), (88.20, 84.80, 88.30) and (6.92%, 7.13%, 6.10%), respectively. The fat content in the muscle of three grouper was 3.0%, 4.2% and 2.4%, respectively; and the content of DHA+EPA in E. fuscoguttatus♀× E. lanceolatus♂ was 24.02%, the content of DHA+EPA in E. fuscoguttatus♀× E. tukula♂ was 23.56%, the content of DHA+EPA in E. fuscoguttatus was 22.76%. There are abundant mineral elements in the muscles of three grouper; five major elements (K, Ca, Na, Mg and P) and five trace elements (Fe, Mn, Cu, Zn and Se) were detected. The research shows that E. fuscoguttatus, E. fuscoguttatus♀× E. tukula♂ and E. fuscoguttatus♀× E. lanceolatus♂ can provide abundant protein, fat, inorganic salt and mineral elements for humans, thus the three groupers are excellent mariculture varieties with high edible value. Moreover, some nutritional indexes of the hybrid E. fuscoguttatus♀× E. tukula♂ was significantly higher than the female parent E. fuscoguttatus.