Effects of sample size and loci number on genetic diversity index in <i>Macrobrachium rosenbergii</i> with microsatellite markers

DAI Xilin; LIU Jie; LI Jingjing; JIANG Zongbing; MING Lei; JIANG Fei

doi:10.11964/jfc.20160410360

Volume 41 Issue 7

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Journal of fisheries of china > 2017 > 41(7): 1083-1095. > DOI: 10.11964/jfc.20160410360

DAI Xilin, LIU Jie, LI Jingjing, JIANG Zongbing, MING Lei, JIANG Fei. Effects of sample size and loci number on genetic diversity index in Macrobrachium rosenbergii with microsatellite markers[J]. Journal of fisheries of china, 2017, 41(7): 1083-1095. DOI: 10.11964/jfc.20160410360

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Effects of sample size and loci number on genetic diversity index in Macrobrachium rosenbergii with microsatellite markers

1.
College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China
2.
Shencao Special Aquatic Product Development Company, Shanghai 201506, China

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Agriculture Research System of Shanghai (201505)

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Corresponding author:
DAI Xilin. E-mail: xldai@shou.edu.cn
Received Date: April 13, 2016
Revised Date: May 22, 2016
Available Online: November 26, 2017
Published Date: June 30, 2017

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Abstract

Abstract

As assessment of genetic structure of population is the key point of aquatic animal breeding, the selection of sample size and loci number is the first step of correct evaluation of population genetic structure. Here, we solved the problem from an analysis of genetic diversity of three breeding groups of Macrobrachium rosenbergii at 60 polymorphic microsatellite loci. The results show that selection of different sample sizes and loci numbers both had a great effect on the genetic diversity index. The sample size was positively correlated with the average number of alleles and the average effective number of alleles, and the sample size was moderate and highly correlated with heterozygosity and Nei′s genetic diversity index, respectively. Only when the sample size exceeds 30, they tend to be stable. When we select the loci, we found the different polymorphism information contents also directly affect the accuracy of the genetic parameters. When the loci number was 5–25, there was a significant difference in the genetic diversity index(P<0.05). When the loci number was more than 25, the genetic parameters changed little(P>0.05). So, we suggested that when microsatellite markers were used to estimate genetic diversity of population as far as possible to choose high polymorphism information content and we can′t consider sample gender, meanwhile the minimum loci number and sample size are needed to exceed 25 and 30, respectively.
- Macrobrachium rosenbergii,
- genetic diversity,
- microsatellite DNA marker,
- sample size

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Effects of sample size and loci number on genetic diversity index in Macrobrachium rosenbergii with microsatellite markers

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