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Volume 42 Issue 3
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SUN Mingyu, FENG Bo, ZHANG Zhaohuan, XU Majunkun, LIU Weiqi, LIU Haiquan, PAN Yingjie, ZHAO Yong. New sequence type isolates of AHPND-causing Vibrio parahaemolyticus from Litopenaeus vannamei by multilocus sequence typing[J]. Journal of fisheries of china, 2018, 42(3): 410-418. DOI: 10.11964/jfc.20170510847
Citation: SUN Mingyu, FENG Bo, ZHANG Zhaohuan, XU Majunkun, LIU Weiqi, LIU Haiquan, PAN Yingjie, ZHAO Yong. New sequence type isolates of AHPND-causing Vibrio parahaemolyticus from Litopenaeus vannamei by multilocus sequence typing[J]. Journal of fisheries of china, 2018, 42(3): 410-418. DOI: 10.11964/jfc.20170510847
shu

New sequence type isolates of AHPND-causing Vibrio parahaemolyticus from Litopenaeus vannamei by multilocus sequence typing

  • 1.

    College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China

  • 2.

    Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai Ocean University, Shanghai    201306, China

  • 3.

    Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai Ocean University, Shanghai    201306, China

  • 4.

    Engineering Research Center of Food Thermal-processing Technology, Shanghai Ocean University, Shanghai    201306, China

Funds: 

National Natural Science Foundation of China (31571917, 31671779); The Project of Science and Technology Commission of Shanghai Municipality (14320502100); Shanghai Agriculture Applied Technology Development Program (G20150408, G20160101, T20170404); Innovation Program of Shanghai Municipal Education Commission (2017-01-07-00-10-E00056); The “Dawn” Program of Shanghai Education Commission (15SG48)

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  • Corresponding author:

    ZHAO Yong. E-mail: yzhao@shou.edu.cn

  • Received Date: May 22, 2017
  • Revised Date: August 22, 2017
  • Available Online: March 05, 2018
  • Published Date: February 28, 2018
    Graphical Abstract
    • Abstract
  • Twelve strains of pathogenic Vibrio parahaemolyticus isolated from Litopenaeus vannamei with acute hepatopancreatic necrosis disease (AHPND) were chosen, and their genetic characteristics were analyzed by the multilocus sequence typing method. Seven house-keeping genes (dnaE, gyrB, recA, dtdS, pntA, pyrC and tnaA) of V. parahaemolyticus were selected as target genes for PCR amplification and nucleic acid sequencing. The sequences of AHPND-causing V. parahaemolyticus in this study were uploaded to the PubMLST database for multi-alignment to determine the sequence type of each strain. MLST data of AHPND-causing V. parahaemolyticus from other areas were collected and the genetic evolution was analyzed by MEGA 5.0 and eBURST V3 software. AHPND-causing V. parahaemolyticus isolated from Guangdong province in 2014 belonged to the new sequence type ST1710(42, 134, 99, 79, 141, 41, 51). Compared with other sequence types in PubMLST database, it has the high homology with ST415 and ST975. The 9 STs of AHPND-causing V. parahaemolyticus were separated by eBURST into two doublets and five singletons. Phylogenetic tree analysis showed that the new sequence type ST1710 was similar to the singletons ST975. Our study is the first report of molecular epidemiology of AHPND-causing V. parahaemolyticus strains in China using multilocus sequence typing, and enriches the PubMLST database and provides an insight into their evolutionary mechanisms.
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    • References (24)
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