Characteristics of formation related genes (<i>PyMFG</i>) of monospores and analysis of differential expression in <i>Pyropia yezoensis</i>

SONG Shanshan; DING Hongchang; YAN Xinghong

doi:10.11964/jfc.20190311710

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Journal of fisheries of china > 2020 > In Press > DOI: 10.11964/jfc.20190311710

SONG Shanshan, DING Hongchang, YAN Xinghong. Characteristics of formation related genes (PyMFG) of monospores and analysis of differential expression in Pyropia yezoensis[J]. Journal of fisheries of china. DOI: 10.11964/jfc.20190311710

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Characteristics of formation related genes (PyMFG) of monospores and analysis of differential expression in Pyropia yezoensis

1.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China
2.
National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
3.
Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China

Funds: National Key Research and Development Program of China (2018YFD0900606); National Natural Science Foundation of China (31072208); Major Science and Technology Specific Program of Zhejiang Province (2016C02055-6); Science and Tecnology Planning Project of Jiangsu Province, China (BE2018335); Open Program of Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province (2017fjscq02)

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Corresponding author:
YAN Xinghong. E-mail: xhyan@shou.edu.cn
Received Date: March 25, 2019
Revised Date: May 27, 2019
Available Online: June 15, 2020

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Abstract

In our study, a complete open reading frame of PyMFG was obtained by RT-PCR based on the sequence of differentially expressed genes (Contig-21827) screened by transcriptome sequencing of Pyropia yezoensis. Sequence analysis showed that the ORF of the PyMFG was 1 224 bp in length and encoded a polypeptide fragment of 407 amino acids with a molecular weight of 46.24 ku, and theoretical pI of 9.08. Domain analysis revealed that the protein contains a conserved TEA domain and a YAP domain, which belongs to the TEA-ATTS superfamily domain. We found that the protein and fungal conidia forming proteins clustered into one large branch with close genetic relationship through multiple sequence alignment and phylogenetic analysis. In addition, the comparison of the ability of releasing monospores and qRT-PCR analysis between different strains indicated that the expression trend of PyMFG in PY26W and PY26W' was highly consistent with macroscopic statistical results, while the trend in PY26R and PY26R' were exactly existed difference. By inference, the gene type and molecular mechanism regulating the formation and release of monospores in the parental (PY26W) and partial parental strain (PY26W') and the female parental (PY26R) as well as partial female strain (PY26R') existed certain difference, leading to the divergence between the macroscopic statistical results of monospores and the qRT-PCR results.
- Pyropia yezoensis,
- RNA-Seq,
- monospores,
- PyMFG,
- TEA-ATTS superfamily,
- qRT-PCR

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Characteristics of formation related genes (PyMFG) of monospores and analysis of differential expression in Pyropia yezoensis

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