SHEN Haiyang, LU Zhijie, QIN Zhendong, ZHAO Lijuan, LI Yanan, LIU Chun, YANG Guang, YE Chengkai, PAN Gan, LIN Li. Relationship between the expression of C-Myc protein and the replication of nervous necrosis virus in GF-1 cells treated with glutamine[J]. Journal of fisheries of china, 2020, 44(9): 1385-1396. DOI: 10.11964/jfc.20190911969
Citation: SHEN Haiyang, LU Zhijie, QIN Zhendong, ZHAO Lijuan, LI Yanan, LIU Chun, YANG Guang, YE Chengkai, PAN Gan, LIN Li. Relationship between the expression of C-Myc protein and the replication of nervous necrosis virus in GF-1 cells treated with glutamine[J]. Journal of fisheries of china, 2020, 44(9): 1385-1396. DOI: 10.11964/jfc.20190911969

Relationship between the expression of C-Myc protein and the replication of nervous necrosis virus in GF-1 cells treated with glutamine

  • In order to investigate the relationships among the C-Myc expression, glutamine metabolism and replication of NNV, C-Myc gene (GF-1-C-Myc) from Epinephelus coioides fin cell (grouper fin cells, GF-1) was cloned. The full length of GF-1-C-Myc gene cDNA was 814 bp with 285 bp ORF, encoding 95 amino acid (aa) with leucine zipper domain and helix-ring-helix (HLH) domain. GF-1-C-Myc protein was expressed and purified, and its polyclonal antibody was generated. The expressions of GF-1-C-Myc gene and the replication of NNV were monitored by real-time quantitative PCR (qRT-PCR) and immunoblotting (WB). The results showed that lack of glutamine could inhibit both the expression of GF-1-C-Myc gene and replication of NNV, while addition of glutamine could promote both the expression of GF-1-C-Myc gene and replication of NNV. In addition, the expression of GF-1-C-Myc gene was up-regulated in GF-1 cells infected with NNV, and the glutamine in the medium was significantly consumed. Taken together, GF-1-C-Myc gene was involved in the regulation of glutamine metabolism in the cell, subsequently facilitated the replication of NNV. Our results will shed a new light on the prevention and control of NNV infection.
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