• ISSN 1000-0615
  • CN 31-1283/S
Volume 44 Issue 9
Sep.  2020
Turn off MathJax
Article Contents
Abstract
References
SHEN Haiyang, LU Zhijie, QIN Zhendong, ZHAO Lijuan, LI Yanan, LIU Chun, YANG Guang, YE Chengkai, PAN Gan, LIN Li. Relationship between the expression of C-Myc protein and the replication of nervous necrosis virus in GF-1 cells treated with glutamine[J]. Journal of fisheries of china, 2020, 44(9): 1385-1396. DOI: 10.11964/jfc.20190911969
Citation: SHEN Haiyang, LU Zhijie, QIN Zhendong, ZHAO Lijuan, LI Yanan, LIU Chun, YANG Guang, YE Chengkai, PAN Gan, LIN Li. Relationship between the expression of C-Myc protein and the replication of nervous necrosis virus in GF-1 cells treated with glutamine[J]. Journal of fisheries of china, 2020, 44(9): 1385-1396. DOI: 10.11964/jfc.20190911969
shu

Relationship between the expression of C-Myc protein and the replication of nervous necrosis virus in GF-1 cells treated with glutamine

  • 1.

    Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou    510631, China

  • 2.

    Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Science & Tecgnology, Zhongkai University of Agriculture and Engineering, Guangzhou    510225, China

Funds: National Natural Science Foundation of China (31872606); Foundation of Guangdong Provincial Marine and Fisheries Bureau (GDME-2018C006, D21822202); Foundation of China-ASEAN Maritime Cooperation (CAMC-2018F); Guangdong Provincial Special Fund For Modern Agriculture Industry Technology Innovation Teams (2019KJ141)
More Information
  • Corresponding author:

    PAN Gan. E-mail: pg2829@sina.com

    LIN Li. E-mail: linli@zhku.edu.cn

  • Received Date: September 16, 2019
  • Revised Date: October 24, 2019
  • Available Online: February 19, 2020
  • Published Date: August 31, 2020
    Graphical Abstract
    • Abstract
  • In order to investigate the relationships among the C-Myc expression, glutamine metabolism and replication of NNV, C-Myc gene (GF-1-C-Myc) from Epinephelus coioides fin cell (grouper fin cells, GF-1) was cloned. The full length of GF-1-C-Myc gene cDNA was 814 bp with 285 bp ORF, encoding 95 amino acid (aa) with leucine zipper domain and helix-ring-helix (HLH) domain. GF-1-C-Myc protein was expressed and purified, and its polyclonal antibody was generated. The expressions of GF-1-C-Myc gene and the replication of NNV were monitored by real-time quantitative PCR (qRT-PCR) and immunoblotting (WB). The results showed that lack of glutamine could inhibit both the expression of GF-1-C-Myc gene and replication of NNV, while addition of glutamine could promote both the expression of GF-1-C-Myc gene and replication of NNV. In addition, the expression of GF-1-C-Myc gene was up-regulated in GF-1 cells infected with NNV, and the glutamine in the medium was significantly consumed. Taken together, GF-1-C-Myc gene was involved in the regulation of glutamine metabolism in the cell, subsequently facilitated the replication of NNV. Our results will shed a new light on the prevention and control of NNV infection.
    • FullText(HTML)
    • References (49)
  • [1]
    黄文, 陈偿, 赵哲, 等. 海水养殖鱼类病毒性神经坏死病防控技术研究进展[J]. 渔业研究, 2016, 38(5): 419-426.

    Huang W, Chen C, Zhao Z, et al. Review on disease control researches of marine fish NNV[J]. Journal of Fisheries Research, 2016, 38(5): 419-426(in Chinese).
    [2]
    陈文捷, 刘晓丹, 胡先勤, 等. 鱼类神经坏死病毒研究进展与发展趋势[J]. 水产学报, 2014, 38(9): 1666-1672.

    Chen W J, Liu X D, Hu X Q, et al. Trend and research progress of nervous necrosis virus[J]. Journal of Fisheries of China, 2014, 38(9): 1666-1672(in Chinese).
    [3]
    粟子丹, 李晋, 史成银. 引起半滑舌鳎(Cynoglossus semilaevis Günther)鱼苗大规模死亡的神经坏死病毒病[J]. 渔业科学进展, 2016, 37(4): 110-115. doi: 10.11758/yykxjz.20150610001

    Su Z D, Li J, Shi C Y. Preliminary study on massive mortality of hatchery-reared half-smooth tongue sole, Cynoglossus semilaevis, associated with viral nervous necrosis[J]. Progress in Fishery Sciences, 2016, 37(4): 110-115(in Chinese). doi: 10.11758/yykxjz.20150610001
    [4]
    陈晓艳. 鱼类病毒性神经坏死病研究现状[J]. 动物医学进展, 2005, 26(5): 17-21. doi: 10.3969/j.issn.1007-5038.2005.05.005

    Chen X Y. Advances in nervous necrosis virus of fish[J]. Progress in Veterinary Medicine, 2005, 26(5): 17-21(in Chinese). doi: 10.3969/j.issn.1007-5038.2005.05.005
    [5]
    刘荭, 史秀杰, 高隆英, 等. 鱼病毒性神经坏死病病毒(VNNV)不同基因型鉴别方法的建立及在VNN检疫和监测中的应用[J]. 水产学报, 2004, 28(6): 695-702.

    Liu H, Shi X J, Gao L Y, et al. Establishment of discriminating method of various genotypes of viral nervous necrosis virus (VNNV) and application in quarantine and surveillance of VNNV[J]. Journal of Fisheries of China, 2004, 28(6): 695-702(in Chinese).
    [6]
    陈晓艳, 何建国. 原位杂交技术在斜带石斑鱼神经坏死病毒检测中的应用[J]. 海洋科学, 2008, 32(6): 1-4.

    Chen X Y, He J G. Application of in situ hybridization in testing orange-sported grouper nervous necrosis virus[J]. Marine Sciences, 2008, 32(6): 1-4(in Chinese).
    [7]
    陈晓艳, 翁少萍, 吕玲, 等. 斜带石斑鱼神经坏死病毒基因组RNA1和RNA2序列测定及分析[J]. 中山大学学报(自然科学版), 2005, 44(1): 73-77.

    Chen X Y, Weng S P, Lü L, et al. Sequences of RNA1 and RNA2 from orange-spotted grouper (Epinephelus coioids) nervous necrosis virus, China Strain[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2005, 44(1): 73-77(in Chinese).
    [8]
    陈晓艳, 翁少萍, 殷志新, 等. 斜带石斑鱼神经坏死病毒主衣壳蛋白的原核表达与纯化[J]. 中山大学学报(自然科学版), 2005, 44(6): 83-86. doi: 10.3321/j.issn:0529-6579.2005.06.022

    Chen X Y, Weng S P, Yin Z X, et al. Expression and purification of major capsid protein of orange-spotted grouper nervous necrosis virus[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2005, 44(6): 83-86(in Chinese). doi: 10.3321/j.issn:0529-6579.2005.06.022
    [9]
    黄桂菊, 陈健光, 喻达辉, 等. 斜带石斑鱼神经坏死病毒CP基因shRNA干扰载体的构建及效果评价[J]. 水产学报, 2012, 36(3): 343-349. doi: 10.3724/SP.J.1231.2012.27714

    Huang G J, Chen J G, Yu D H, et al. Construction and evaluation of short hairpin RNA interference vector targeting CP gene of Epinephelus coioides nervous necrosis virus[J]. Journal of Fisheries of China, 2012, 36(3): 343-349(in Chinese). doi: 10.3724/SP.J.1231.2012.27714
    [10]
    林克冰, 方琼珊, 吴建绍, 等. 石斑鱼神经坏死病毒传播途径阻断的初步研究[J]. 福建水产, 2011, 33(5): 15-19.

    Lin K B, Fang Q S, Wu J S, et al. Preliminary study on blocking transmission of NNV in Epinephelus coioides[J]. Journal of Fujian Fisheries, 2011, 33(5): 15-19(in Chinese).
    [11]
    翟伟锋, 殷波. 石斑鱼神经坏死病毒的RT-LAMP检测方法的建立[J]. 南方农业, 2017, 11(32): 67-69.

    Zhai W F, Yin B. Establishment of RT-LAMP method for detection of Epinephelus coioides nerve necrosis virus[J]. South China Agriculture, 2017, 11(32): 67-69(in Chinese).
    [12]
    葛辉, 林克冰, 周宸, 等. 10种免疫相关基因在斜带石斑鱼组织中的表达分析[J]. 渔业研究, 2017, 39(5): 379-385.

    Ge H, Lin K B, Zhou C, et al. mRNA expression of ten immune genes in tissues of Epinephelus coioides[J]. Journal of Fisheries Research, 2017, 39(5): 379-385(in Chinese).
    [13]
    林蠡, 黄剑南, 翁少萍, 等. 赤点石斑鱼病毒性神经坏死症的组织病理和电镜观察[J]. 水产学报, 2005, 29(4): 519-523.

    Lin L, Huang J N, Weng S P, et al. Histopathological examination and electron microscopy observation of viral nervous necrosis in red-spotted grouper, Epinephelus akaara[J]. Journal of Fisheries of China, 2005, 29(4): 519-523(in Chinese).
    [14]
    Hegde A, Teh H C, Lam T J, et al. Nodavirus infection in freshwater ornamental fish, guppy, Poicelia reticulata– comparative characterization and pathogenicity studies[J]. Archives of Virology, 2003, 148(3): 575-586. doi: 10.1007/s00705-002-0936-x
    [15]
    朱国华. NNV来源的蛋白转导域入侵机制的研究[D]. 广州: 中山大学, 2017.

    Zhu G H. Study of the entry mechanism of protein transduction domain originated from nervous necrosis virus[D]. Guangzhou: Sun Yat-sen University, 2017(in Chinese).
    [16]
    伊丽竹. 抗鱼类神经坏死病毒和抗嗜水气单胞菌卵黄抗体的制备及特性分析[D]. 武汉: 华中农业大学, 2018.

    Yi L Z. Production and features of egg yolkigy against red-spotted grouper nervous necrosis virus and Aeromonas hydrophila[D]. Wuhan: Huazhong Agricultural University, 2018(in Chinese).
    [17]
    张成香. c-myc转基因小鼠生物学特性的研究[D]. 扬州: 扬州大学, 2006.

    Zhang C X. Study of biological character of c-myc transgenic mice model[D]. Yangzhou: Yangzhou University, 2006(in Chinese).
    [18]
    孙慧慧. C-Myc单克隆抗体的制备及鉴定[D]. 大连: 辽宁师范大学, 2011.

    Sun H H. Preparation and identification of c-myc monoclonal antibody[D]. Dalian: Liaoning Normal University, 2011(in Chinese).
    [19]
    Lucas B, Grigo K, Erdmann S, et al. HNF4α reduces proliferation of kidney cells and affects genes deregulated in renal cell carcinoma[J]. Oncogene, 2005, 24(42): 6418-6431. doi: 10.1038/sj.onc.1208794
    [20]
    Chang D W, Claassen G F, Hann S R, et al. The C-Myc transactivation domain is a direct modulator of apoptotic versus proliferative signals[J]. Molecular and Cellular Biology, 2000, 20(12): 4309-4319. doi: 10.1128/MCB.20.12.4309-4319.2000
    [21]
    Shon W, Sukov W R, Jenkins S M, et al. MYC amplification and overexpression in primary cutaneous angiosarcoma: a fluorescence in-situ hybridization and immunohistochemical study[J]. Modern Pathology, 2014, 27(4): 509-515. doi: 10.1038/modpathol.2013.163
    [22]
    赵晓航. c-myc基因表达的急剧抑制导致人食管癌细胞系的终末分化和凋亡[D]. 北京: 中国协和医科大学, 1993.

    Zhao X H. The rapid inhibition of c-myc gene expression leads to terminal differentiation and apoptosis of human esophageal cancer cell line[D]. Beijing: Peking Union Medical College, 1993(in Chinese).
    [23]
    Fernandez P C, Frank S R, Wang L Q, et al. Genomic targets of the human c-Myc protein[J]. Genes & Development, 2003, 17(9): 1115-1129.
    [24]
    Wang C G, Tai Y H, Lisanti M P, et al. c-Myc induction of programmed cell death may contribute to carcinogenesis[J]. Cancer Biology & Therapy, 2011, 11(7): 615-626.
    [25]
    赵冶. 通过反转录病毒侵染建立稳定过表达C-Myc的MIN6细胞系[D]. 哈尔滨: 东北林业大学, 2011.

    Zhao Y. Establishment of min6 cell line with stable over expression of c-Myc through retrovirus infection[D]. Harbin: Northeast Forestry University, 2011(in Chinese).
    [26]
    Battey J, Moulding C, Taub R, et al. The human c-myc oncogene: structural consequences of translocation into the igh locus in Burkitt lymphoma[J]. Cell, 1983, 34(3): 779-787. doi: 10.1016/0092-8674(83)90534-2
    [27]
    Scott N, Martin I, Jack A S, et al. Genes mediating programmed cell death: an immunohistochemical study of bcl-2, c-myc and p53 expression in colorectal neoplasia[J]. Clinical Molecular Pathology, 1996, 49(3): M151-M158. doi: 10.1136/mp.49.3.M151
    [28]
    Bissonnette R P, Echeverri F, Mahboubi A, et al. Apoptotic cell death induced by c-myc is inhibited by bcl-2[J]. Nature, 1992, 359(6395): 552-554. doi: 10.1038/359552a0
    [29]
    林超辉. 斜带石斑鱼的生物学特性及养殖技术[J]. 北京水产, 2007(5): 14-16.

    Lin C H. Biological characteristics and culture techniques of Epinephelus coioides[J]. Journal of Beijing Fisheries, 2007(5): 14-16(in Chinese).
    [30]
    徐大为, 邢克智, 白东清, 等. 点带石斑鱼肌肉中氨基酸组成分析[J]. 中国水产, 2007(4): 62-64. doi: 10.3969/j.issn.1002-6681.2007.04.038

    Xu D W, Xing K Z, Bai D Q, et al. Analysis of amino acid composition in muscle of Epinephelus coioides punctatus[J]. China Fisheries, 2007(4): 62-64(in Chinese). doi: 10.3969/j.issn.1002-6681.2007.04.038
    [31]
    林克冰, 朱志煌, 葛辉, 等. 斜带石斑鱼(Epinephelus coioides)神经坏死病毒感染过程的免疫研究[C]//福建省海洋学会2016年学术年会论文摘要集. 厦门: 福建省海洋学会, 福建省水产研究所, 2016.

    Lin K B, Zhu Z H, Ge H, et al. Immunity to nervous necrosis virus infections of nge-spotted grouper (Epinephelus coioides) by vaccination with irus-like particles[C]//Annual meeting of Fujian Marine Society 2016. Xiamen: Fujian Marine Society, Fujian Fisheries Research Institute, 2016(in Chinese).
    [32]
    林克冰, 方琼珊, 王涵生, 等. 斜带石斑鱼生态优化与病害防控育苗模式的研究[J]. 福建水产, 2013, 35(5): 368-374. doi: 10.3969/j.issn.1006-5601.2013.05.006

    Lin K B, Fang Q S, Wang H S, et al. Studies on new breeding model of ecological optimization and disease control and prevention in Epinephlus coioides[J]. Journal of Fujian Fisheries, 2013, 35(5): 368-374(in Chinese). doi: 10.3969/j.issn.1006-5601.2013.05.006
    [33]
    Denno R, Rounds J D, Faris R, et al. Glutamine-enriched total parenteral nutrition enhances plasma glutathione in the resting state[J]. Journal of Surgical Research, 1996, 61(1): 35-38. doi: 10.1006/jsre.1996.0077
    [34]
    Passerini L, Welbourne T C. Organic mercurial diuresis: Inhibition of glutamine utilization in the acidotic rat[J]. Canadian Journal of Physiology and Pharmacology, 1979, 57(3): 227-234. doi: 10.1139/y79-035
    [35]
    Welbourne T C, Dass P D. Function of renal γ-glutamyltransferase: significance of glutathione and glutamine interactions[J]. Life Sciences, 1982, 30(10): 793-801. doi: 10.1016/0024-3205(82)90591-4
    [36]
    Dang C V. Rethinking the Warburg effect with myc micromanaging glutamine metabolism[J]. Cancer Research, 2010, 70(3): 859-862. doi: 10.1158/0008-5472.CAN-09-3556
    [37]
    Gao P, Tchernyshyov I, Chang T C, et al. c-Myc suppression of miR-23a/b enhances mitochondrial glutaminase expression and glutamine metabolism[J]. Nature, 2009, 458(7239): 762-765. doi: 10.1038/nature07823
    [38]
    Wise D R, DeBerardinis R J, Mancuso A, et al. Myc regulates a transcriptional program that stimulates mitochondrial glutaminolysis and leads to glutamine addiction[J]. Proceedings of the National Academy of Sciences of the United States of America, 2008, 105(48): 18782-18787. doi: 10.1073/pnas.0810199105
    [39]
    卢志杰, 叶成凯, Sarath B V, 等. 罗氏沼虾谷氨酰胺合成酶基因的克隆与表达[J]. 水产学报, 2019, 43(3): 549-562.

    Lu Z J, Ye C K, Sarath B V, et al. Cloning and expression of glutamine synthetase from giant freshwater prawn (Macrobrachium rosenbergii)[J]. Journal of Fisheries of China, 2019, 43(3): 549-562(in Chinese).
    [40]
    叶成凯, 卢志杰, Sarath B V, 等. 罗氏沼虾几丁质酶3B基因的克隆及其在蜕皮周期中的表达[J]. 水产学报, 2019, 43(4): 751-762.

    Ye C K, Lu Z J, Sarath B V, et al. Cloning and expression analysis of chitinase-3B from giant freshwater prawn (Macrobrachium rosenbergii) during molting cycle[J]. Journal of Fisheries of China, 2019, 43(4): 751-762(in Chinese).
    [41]
    苏国茂, 秦真东, 李嘉波, 等. 罗湖病毒核蛋白的克隆表达、抗体制备及其组织分布[J]. 水产学报, 2019, 43(12): 2040-2053.

    Su G M, Qin Z D, Li J B, et al. Cloning, expression, antibody preparation and tissue distribution of tilapia lake virus nucleoprotein[J]. Journal of Fisheries of China, 2019, 43(12): 2040-2053(in Chinese).
    [42]
    李嘉波, 秦真东, 赵丽娟, 等. 罗非鱼湖病毒对吉富尼罗罗非鱼和E-11细胞的感染[J]. 水产学报, 2020, 44(1): 142-155.

    Li J B, Qin Z D, Zhao L J, et al. Infection of Tilapia Lake virus in Oreochromis niloticus and E-11 cell[J]. Journal of Fisheries of China, 2020, 44(1): 142-155(in Chinese).
    [43]
    Wu G W, Yuan M Q, Shen S Q, et al. Menin enhances c-Myc-mediated transcription to promote cancer progression[J]. Nature Communications, 2017, 8(1): 15278. doi: 10.1038/ncomms15278
    [44]
    Muhammad Asim. 谷氨酰胺对赤点石斑鱼神经坏死病毒复制影响的研究[D]. 武汉: 华中农业大学, 2016.

    Muhammad Asim. Effect of glutamine on nerve necrosis virus replication of Epinephelus akaara [D]. Wuhan: Huazhong Agricultural University, 2016 (in Chinese).
    [45]
    付小哲. 鳜传染性脾肾坏死病毒敏感细胞系的建立及病毒增殖依赖于谷氨酰胺的机制研究[D].杨凌: 西北农林科技大学, 2017.

    Fu X Z. Establishment of sensitive cell line of infectious spleen and kidney necrosis virus in Siniperca chuatsi and study on the mechanism of glutamine-dependent virus proliferation [D]. Yangling: Northwest University of Agriculture and Forestry Science and Technology, 2017 (in Chinese).
    [46]
    郭茜茜, 付小哲, 梁红茹, 等. 鳜弹状病毒调控谷氨酰胺还原性代谢途径促进自身增殖[J]. 中国水产科学, 2019, 26(5): 993-1003.

    Guo X X, Fu X Z, Liang H R, et al. Siniperca chuatsi rhabdovirus regulates glutamine reductive metabolic pathway to promote self-proliferation[J]. Chinese Aquatic Sciences, 2019, 26(5): 993-1003.
    [47]
    Ma R R, Wang Y G, Liao M J, et al. Diagnosis and ORF gene sequencing analysis of the nervous necrosis virus (NNV) isolated from cultured pearl gentian grouper, Epinephelus lanceolatus × Epinephelus fuscoguttatus, in China[C]//Proceedings of the 7th International Conference on Biomedical Engineering and Informatics. Dalian, China: IEEE, 2015.
    [48]
    Thai M, Graham N A, Braas D, et al. Adenovirus E4ORF1-induced MYC activation promotes host cell anabolic glucose metabolism and virus replication[J]. Cell Metabolism, 2014, 19(4): 694-701. doi: 10.1016/j.cmet.2014.03.009
    [49]
    Thai M, Thaker S K, Feng J, et al. MYC-induced reprogramming of glutamine catabolism supports optimal virus replication[J]. Nature Communications, 2015, 6(1): 8873. doi: 10.1038/ncomms9873
    • Related Articles

Catalog

    Get Citation
    PDF in Chinese
    XML
    Article views (3042) PDF downloads (50) Cited by()
    Related

    Website Copyright © Editorial Office of Journal of Fisheries of China

    Address：No. 999, Hucheng Ring Road, Pudong New Area, Shanghai

    Tel：021-61900228 E-mail：jfc@china-fishery.com   

    京ICP备12043220号

    Supported by: Beijing Renhe Information Technology Co. Ltd  support: info@rhhz.net

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return