Comparative analysis of skin and eye transcriptome in the <i>Gobiopterus lacustris</i>

LI Xueyou; XIE Minghua; HUANG Chengqin; WANG Chun; WANG Zhongduo; GUO Yusong; DONG Zhongdian

doi:10.11964/jfc.20200912401

Volume 45 Issue 8

Aug. 2021

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Journal of fisheries of china > 2021 > 45(8): 1317-1326. > DOI: 10.11964/jfc.20200912401

LI Xueyou, XIE Minghua, HUANG Chengqin, WANG Chun, WANG Zhongduo, GUO Yusong, DONG Zhongdian. Comparative analysis of skin and eye transcriptome in the Gobiopterus lacustris[J]. Journal of fisheries of china, 2021, 45(8): 1317-1326. DOI: 10.11964/jfc.20200912401

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Comparative analysis of skin and eye transcriptome in the Gobiopterus lacustris

Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China

Funds: National Natural Science Foundation of China (41806195, 31201996); Technology Planning Project of Guangdong Province, China (2017A030303075); Guangdong Ocean University Featured Innovation Project (230419069, 230419055); Guangdong Ocean University Nanhai Scholars Program Young Talent Project (QNXZ201903, 201807); Start-up Project from GDOU PhD

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Corresponding author:
DONG Zhongdian. E-mail: zddong@gdou.edu.cn
Received Date: September 12, 2020
Revised Date: October 27, 2020
Available Online: March 02, 2021
Published Date: July 31, 2021

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Abstract

Gobiopterus lacustris is a kind of small fish with strong adaptability to salinity and easy to raise in laboratory. It is an ideal model organism and environmental indicator species, whose body is transparent and its organs can be seen through naked eyes. In order to explore the cause of the transparent body color of G. lacustris, the transcriptome analysis of skin and eye of G. lacustris was carried out. A total of 103 686 unigenes were obtained by de novo splicing. The N50 and average length were 1 456 and 2 490 bp, respectively. In Nr, Nt, KO, SwissProt, PFAM, GO and KOG databases, 57 380, 37 343, 31 700, 51 277, 47 020, 47 555 and 25 604 unigenes were annotated respectively. KEGG enrichment showed that tyr, tyrp1 and tyrp2 were significantly down regulated in melanin production pathway. In addition, the results showed that there were 8 113 differentially expressed genes (DEGs) in the skin and eyes of G. lacustris, of which 3 174 were up-regulated in the skin and 4 939 in the eyes. The verification of 10 DEGs by qPCR confirmed that the RNA-Seq analysis was correct. This study enriches the body color research of G. lacustris, provides data for the utilization of G. lacustris genetic resources, and also provides a reference for further study on the formation mechanism of gorgeous body color of fish.
- Gobiopterus lacustris,
- skin,
- eye,
- RNA-Seq,
- pigment formation,
- body color

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Comparative analysis of skin and eye transcriptome in the Gobiopterus lacustris

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