Diversity of microflora involved in skin ulcer and death of <i>Andrias davidianus</i>

XU Junqi; XIE Jianping; WANG Zhijian

doi:10.11964/jfc.20201012441

Volume 46 Issue 11

Nov. 2022

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Journal of fisheries of china > 2022 > 46(11): 2186-2195. > DOI: 10.11964/jfc.20201012441

XU Junqi, XIE Jianping, WANG Zhijian. Diversity of microflora involved in skin ulcer and death of Andrias davidianus[J]. Journal of fisheries of china, 2022, 46(11): 2186-2195. DOI: 10.11964/jfc.20201012441

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Diversity of microflora involved in skin ulcer and death of Andrias davidianus

1.
Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Key Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, Chongqing 400715, China
2.
State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Institute of Modern Biopharmaceuticals, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China

Funds: National Key Research and Development Plan (2016YFC0502304); 2020 Chongqing Postdoctoral Research Project (7820100597)

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Corresponding author:
WANG Zhijian. E-mail: wangzj1969@126.com
Received Date: October 13, 2020
Revised Date: January 07, 2021
Available Online: July 03, 2022
Published Date: October 31, 2022

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Abstract

To explore the dynamics of microflora on diseased and healthy skin or in water samples of Andrias davidianus in natural environment, and provide microbial basis for rational aquaculture, protection of wild A. davidianus and future development of probiotics to improve the breeding of A. davidianus, in this study, the skin microflora of A. davidianus were profiled by high-throughput sequencing of the V4-V5 region of 16S rDNA gene, and the changes of skin microflora were compared between the healthy and the diseased A. davidianus. There were significant differences in the diversity, structure and function between healthy and diseased A. davidianus skin bacteria.The average number of Tag of each sample was 97 150, and the average length of sequencing was 400 bp. The OTU data showed that the microbial abundance of healthy skin was much higher than that of diseased skin. The dominant bacterium in the living water samples of healthy A. davidianus was Aliihoeflea, and after A. davidianus died, it changed to Chryseobacterium. The abundance of Chryseobacterium, Comamonas and Burkholderiales increased significantly, while that of Aliihoeflea, Exiguobacterium and Planococcus rifietoensis decreased significantly (P<0.05). Meanwhile, the skin surface of the dead Andrias davidianus had a large number of ulcers with pili, with typical symptoms of Burkella infection. Our findings confirmed that there were significant differences in skin microflora of dead and diseased A. davidianus. Burkella zoonotic bacteria might be the main cause of the death of A. davidianus in natural environment contaminated by water vector. This provided a basis for scientific breeding of A. davidianus.
- Andrias davidianus,
- pathogen,
- community structure,
- 16S rDNA

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Diversity of microflora involved in skin ulcer and death of Andrias davidianus

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