Cloning and immunological function of SBNO1 gene in Exopalaemon carinicauda

In order to explore the immune function of Strawberry Notch1 gene (SBNO1) in crustaceans, the full length of SBNO1 gene cDNA sequence, phylogeny, tissue distribution, the expression of SBNO1 and related genes in Notch signaling pathways by Vibrio parahaemolyticus (VP) injection were studied in this paper. The results showed that the cDNA sequence of SBNO1 was 4 353 bp, encoding a total of 1380 amino acids. The predicted molecular weight of protein is 158.91 ku and theoretical isoelectric point is 4.8. According to the RT-qPCR analysis, it can be found that SBNO1 was expressed in all tested tissues, among which hepatopancreas had the highest level and gonad followed. Futhermore, after RNAi interference, compared with the non-inference group, the death rate of the inference group injected with VP was significantly higher. The transcription of SBNO1, Numb and Delta in the hepatopancreas in Notch signaling was obviously influenced by VP. However, there was no significant difference in the expression of Jagged. In the group injected with VP, the expression levels of SBNO1 and Numb increased first and then decreased over time, while the expression of Delta decreased first and then increased. The results indicate that Notch signaling pathway plays an important role in the immunity of E. carinicauda by VP injection. This study helps to clarify the mechanism of invertebrate innate immunity and provides a theoretical basis for molecular breeding of E. carinicauda.