• ISSN 1000-0615
  • CN 31-1283/S
LI Yinkang, ZHONG Jinxiang, BAO Yuyuan, YING Ziwei, XIE Xiaoyong. Path analysis and allometric growth of morphological characters and body mass of juvenile Tachypleus tridentatus[J]. Journal of fisheries of china, 2023, 47(4): 049110. DOI: 10.11964/jfc.20210412790
Citation: LI Yinkang, ZHONG Jinxiang, BAO Yuyuan, YING Ziwei, XIE Xiaoyong. Path analysis and allometric growth of morphological characters and body mass of juvenile Tachypleus tridentatus[J]. Journal of fisheries of china, 2023, 47(4): 049110. DOI: 10.11964/jfc.20210412790

Path analysis and allometric growth of morphological characters and body mass of juvenile Tachypleus tridentatus

Funds: Natural Science Foundation of Hainan Province (322MS154); Special Fund for Basic Scientific Research Business Expenses of Central Public Welfare Research Institutes of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (2019TS21)
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  • Corresponding author:

    XIE Xiaoyong. E-mail: xyxie@scsfri.ac.cn

  • Received Date: April 27, 2021
  • Revised Date: August 08, 2021
  • Available Online: March 19, 2023
  • Published Date: March 31, 2023
  • The blood of the horseshoe crab, an ancient marine arthropod, is widely used in the production of tachypleus amebocyte lysate (TAL) for medical testing. The number of horseshoe crab resources in China has declined significantly in the last 30 years. The study of the relationship between morphological traits and body mass of Tachypleus tridentatus and the analysis of anisotropic growth will not only help to understand the growth characteristics of juvenile T. tridentatus, but also reflect the resource allocation strategy and help to conserve horseshoe crab resources. In this study, the cephalothorax length (X1), cephalothorax width (X2), opisthosoma length (X3), opisthosoma width (X4), telson length(X5), total length (X6) and body mass (Y) of 2-4 instars juvenile T. tridentatus were measured, correlation analysis and path analysis were used to explore the effect of morphological traits on body weight. The results showed that the morphological traits with the highest correlation coefficient (r) of body weight of 2-4 instars juvenile T. tridentatus were cephalothorax width (X2). Path analysis showed that the cephalothorax width (X2) had the most immediate impact on the body weight of 2 and 3 instars juvenile T. tridentatus, and the opisthosoma width (X4) had the greatest direct impact on the body weight of 4 instars juvenile T. tridentatus. The determination analysis showed that the cephalothorax width (X2) had the largest determination coefficient for the body weight of 2 and 3 instars juvenile T. tridentatus, and the opisthosoma width (X4) had the largest determination coefficient for the body mass of 4 instars juvenile T. tridentatus. The determination analysis was consistent with the path analysis. The cephalothorax width and body weight of 2-4 instars T. tridentatus are processed logarithmically.The allometric growth equation was obtained: y=−1.095+2.746x, and the regression coefficient is 2.746(b > 1). This shows that the growth rate of juvenile horseshoe crab body mass is greater than the growth rate of cephalothorax width at this stage, and it is at the stage of positive growth. The present study shows that the morphological traits of seedling screening of T. tridentatus at different age stages are distinct, and the evaluation of the germplasm of the juvenile T. tridentatus should take into account its allometric growth pattern. The current study also provides a basis for exploring the relationship between growth and traits, structure and physiology of T. tridentatus.
  • [1]
    鲍虞园, 叶国玲, 颉晓勇. 中国鲎人工繁育及1龄稚鲎形态性状对体质量的影响[J]. 渔业科学进展, 2020, 41(4): 77-84.

    Bao Y Y, Ye G L, Xie X Y. Effects of morphological characters on body weight of artificially bred the first instars of horseshoe crabs (Tachypleus tridentatus)[J]. Progress in Fishery Sciences, 2020, 41(4): 77-84 (in Chinese).
    [2]
    颉晓勇, 钟金香, 关杰耀, 等. 基于鲎试剂需求的中国鲎资源保护的迫切性研究[J]. 中国渔业经济, 2021, 39(2): 109-116.

    Xie X Y, Zhong J X, Guan J Y, et al. The urgency of horseshoe crab protection in China from the perspective of Tachypleus Amebocyte Lysate industry[J]. Chinese Fisheries Economics, 2021, 39(2): 109-116 (in Chinese).
    [3]
    Liao Y Y, Hsieh H L, Xu S Q, et al. Wisdom of crowds reveals decline of Asian horseshoe crabs in Beibu Gulf, China[J]. Oryx, 2019, 53(2): 222-229. doi: 10.1017/S003060531700117X
    [4]
    Laurie K, Chen C P, Cheung, S G, et al. Tachypleus tridentatus[EB/OL]. The IUCN Red List of Threatened Species 2019: e. T21309A149768986. [2020-08-22]. https://dx.doi.org/10.2305/IUCN.UK.20191.RLTS.T21309A149768986.en.
    [5]
    国家林业和草原局. 国家重点野生动物名录[N]. 2021-02-05.

    National Forestry and Grassland Administration. List of national key wildlife[N]. 2021-02-05 (in Chinese).
    [6]
    Hsieh H L, Chen C P. Current status of Tachypleus tridentatus in Taiwan for red list assessment[M]//Carmichael R, Botton M, Shin P, et al. Changing Global Perspectives on Horseshoe Crab Biology, Conservation and Management. Cham: Springer, 2015: 383-396.
    [7]
    Nossa C W, Havlak P, Yue J X, et al. Joint assembly and genetic mapping of the Atlantic horseshoe crab genome reveals ancient whole genome duplication[J]. GigaScience, 2014, 3(1): 9. doi: 10.1186/2047-217X-3-9
    [8]
    Carmichael R H, Rutecki D, Valiela I. Abundance and population structure of the Atlantic horseshoe crab Limulus polyphemus in Pleasant Bay, Cape Cod[J]. Marine Ecology Progress Series, 2003, 246: 225-239. doi: 10.3354/meps246225
    [9]
    Tanacredi J T. Horseshoe crabs imperiled?[M]//Tanacredi J T. Limulus in the Limelight. Boston, MA: Springer, 2002: 7-16.
    [10]
    张嘉丽, 王庆恒, 邓岳文, 等. 斧文蛤(Meretrix Lamarchii)形态性状对体质量的影响效果分析[J]. 渔业科学进展, 2014, 35(6): 110-113. doi: 10.11758/yykxjz.20140616

    Zhang J L, Wang Q H, Deng Y W, et al. Effects of morphological traits on the weight of Meretrix lamarchii[J]. Progress in Fishery Sciences, 2014, 35(6): 110-113 (in Chinese). doi: 10.11758/yykxjz.20140616
    [11]
    陈秀荔, 罗玲娟, 彭敏, 等. 圆尾鲎与中国鲎异速生长研究[J]. 西南农业学报, 2017, 30(1): 238-344.

    Chen X L, Luo L J, Peng M, et al. Allometric growth of Carcinoscorpius rotundicauda and Tachypleus tridentatus[J]. Southwest China Journal of Agricultural Sciences, 2017, 30(1): 238-344 (in Chinese).
    [12]
    邹丽珍. 中国鲎异速生长方程的建立及校验[J]. 福建农业学报, 2018, 33(8): 787-793. doi: 10.19303/j.issn.1008-0384.2018.08.004

    Zou L Z. Establishment and validation of allometric growth models for Tachypleus tridentatus[J]. Fujian Journal of Agricultural Sciences, 2018, 33(8): 787-793 (in Chinese). doi: 10.19303/j.issn.1008-0384.2018.08.004
    [13]
    贺纪正, 曹鹏, 郑袁明. 代谢异速生长理论及其在微生物生态学领域的应用[J]. 生态学报, 2013, 33(9): 2645-2655. doi: 10.5846/stxb201202080164

    He J Z, Cao P, Zheng Y M. Metabolic scaling theory and its application in microbial ecology[J]. Acta Ecologica Sinica, 2013, 33(9): 2645-2655 (in Chinese). doi: 10.5846/stxb201202080164
    [14]
    李春萍, 李刚, 肖春旺. 异速生长关系在陆地生态系统生物量估测中的应用[J]. 世界科技研究与发展, 2007, 29(2): 51-57. doi: 10.3969/j.issn.1006-6055.2007.02.010

    Li C P, Li G, Xiao C W. The application of allometric relationships in biomass estimation in terrestrial ecosystems[J]. World Sci-Tech R & D, 2007, 29(2): 51-57 (in Chinese). doi: 10.3969/j.issn.1006-6055.2007.02.010
    [15]
    许友卿, 刘伟茹, 陈秀荔, 等. 10龄及14龄中国鲎和圆尾鲎头胸甲宽度与体重的回归分析[J]. 水生态学杂志, 2014, 35(3): 70-77. doi: 10.3969/j.issn.1674-3075.2014.03.012

    Xu Y Q, Liu W R, Chen X L, et al. Regression analysis between prosomal width and body weight of 10th and 14th instar stages of Tachypleus tridentatus and Carcinoscorpius rotundicauda[J]. Journal of Hydroecology, 2014, 35(3): 70-77 (in Chinese). doi: 10.3969/j.issn.1674-3075.2014.03.012
    [16]
    Huo Z M, Yan X W, Zhao L Q, et al. Effects of shell morphological traits on the weight traits of Manila clam (Ruditapes philippinarum)[J]. Acta Ecologica Sinica, 2010, 30(5): 251-256. doi: 10.1016/j.chnaes.2010.08.004
    [17]
    李莉, 王雪, 菅玉霞, 等. 不同月龄大泷六线鱼形态性状与体质量的相关性及通径分析[J]. 上海海洋大学学报, 2019, 28(1): 58-66.

    Li L, Wang X, Jian Y X, et al. Correlation and path analysis between morphological traits and body mass of Hexagrammos otakii at different months of age[J]. Journal of Shanghai Ocean University, 2019, 28(1): 58-66 (in Chinese).
    [18]
    薛宝宝, 李浩, 牛东红, 等. 不同月龄缢蛏新品种数量性状的相关与通径分析[J]. 水产学报, 2018, 42(6): 941-949.

    Xue B B, Li H, Niu D H, et al. Correlation and path analysis of quantitative traits of new variety of Sinonovacula constricta at different months of age[J]. Journal of Fisheries of China, 2018, 42(6): 941-949 (in Chinese).
    [19]
    李松岗. 实用生物统计[M]. 北京: 北京大学出版社, 2002: 1-256.

    Li S G. Practical Biostatistics[M]. Beijing: Peking University Press, 2002: 1-256 (in Chinese).
    [20]
    洪水根. 中国鲎生物学研究[M]. 厦门: 厦门大学出版社, 2011: 16-20.

    Hong S G. Biology of horseshoe crabs, Tachypleus tridentatus[M]. Xiamen: Xiamen University Press, 2011: 16-20 (in Chinese).
    [21]
    刘磊, 李健, 高保全, 等. 三疣梭子蟹不同日龄生长性状相关性及其对体重的影响[J]. 水产学报, 2009, 33(6): 964-971.

    Liu L, Li J, Gao B Q, et al. Correlation of growth traits of Portunus trituberculatus at the different ages and its impact on body weight[J]. Journal of Fisheries of China, 2009, 33(6): 964-971 (in Chinese).
    [22]
    李忠炉. 黄渤海小黄鱼、大头鳕和黄鮟种群生物学特征的年际变化[D]. 青岛: 中国科学院海洋研究所, 2011.

    Li Z L. Interannual changes in biological characteristics of small yellow croaker Larimichthys polyactis, Pacific cod Gadus macrocephalus and anglerfish Lophius litulon in the Bohai sea and Yellow Sea[D]. Qingdao: Institute of Oceanology of the Chinese Academy of Sciences, 2011 (in Chinese).
    [23]
    Lee C N, Morton B. Experimentally derived estimates of growth by juvenile Tachypleus tridentatus and Carcinoscorpius rotundicauda (Xiphosura) from nursery beaches in Hong Kong[J]. Journal of Experimental Marine Biology and Ecology, 2005, 318(1): 39-49. doi: 10.1016/j.jembe.2004.12.010
    [24]
    Ismail N, Jolly J J, Dzulkiply S K, et al. Allometric variation of horseshoe crab (Tachypleus gigas) populations collected from Chendor and Cherating, Pahang, peninsular Malaysia[J]. Journal of Sustainability Science and Management, 2012, 7(2): 164-169.
    [25]
    Schreibman M P, Zarnoch C B. Aquaculture methods and early growth of juvenile horseshoe crabs (Limulus polyphemus)[M]//Tanacredi J T, Botton M L, Smith D. Biology and conservation of horseshoe crabs. Boston, MA: Springer, 2009: 501-511.
    [26]
    Chee H Y. Morphometric variations and population size of horseshoe crab (T. gigas) in Teluk Senangin, Perak, Malaysia[J]. American Journal of Emergency Medicine, 2015, 3(4): 379.
    [27]
    刘伟茹. 广西北部湾地区圆尾鲎与中国鲎生物学研究[D]. 南宁: 广西大学, 2014.

    Liu W R. Biological study of Carcinoscorpius rotundicauda and Tachypleus tridentatus in coastal areas of Beibu Gilf in Guangxi[D]. Nanning: Guangxi University, 2014 (in Chinese).
    [28]
    Chatterji A K. Studies on the biology of some carps[D]. Aligarh, India: Aligarh Muslim University, 1976.
    [29]
    Zadeh S S, Christianus A, Saad C R, et al. Comparisons in prosomal width and body weight among early instar stages of Malaysian horseshoe crabs, Carcinoscorpius rotundicauda and Tachypleus gigas in the laboratory[M]//Tanacredi J, Botton M, Smith D. Biology and Conservation of Horseshoe Crabs. Boston, MA: Springer, 2009: 267-274.

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