Citation: | JIANG Peng, FAN Jiajia, LI Shengjie, DU Jinxing, LEI Caixia. Determination and analysis of the amount of mesenteric fat deposition in grass carp (Ctenopharyngodon idella)[J]. Journal of fisheries of china, 2024, 48(2): 029605. DOI: 10.11964/jfc.20211113175 |
Mesenteric fat weight (MFW) is a representative index used to reflect body lipid level in Ctenopharyngodon idella. However, the traditional measurements by artificial scrape and weighing are time-consuming, labor intensive and having high operating errors, especially when many samples are to be handled. To avoid such limitations, this study developed a rapid and sensitive method for the quantitation of mesenteric fat utilizing the lysochrome dye oil red O. The initial operation procedures included: fish anesthetization, sample collection of visceral mass, PIT-tag implantation, sample fixation, sample dehydration, oil red O staining. After the above steps, mesenteric fat could be specifically stained by oil red O dye, and all the samples were stained uniformly. After sample extraction and absorbance measurement of extraction liquid, the weight of oil red O extracted from the stained sample could be accurately obtained according to the standard curve of oil red O (y = 0.0276x + 0.0403, R2 = 0.999 7). The oil red O weights were used as MFWs in this study, and had good consistency with the fat weights quantified by the conventional scraping method (r = 0.80). Furthermore, data analysis showed that the coefficient of variation for MFW (24.49%) was greater than that for body weight (8.93%) in the cultured population of C. idella (n = 200), indicating a significant potential for its genetic improvement. Correlation and cluster analysis showed that MFW was positively correlated with all the observed traits, of which visceral weight (VW) had the highest correlation coefficient of 0.60 (P ˂ 0.01), and was clustered into one group. The results were as expected that both MFW and VW belonged to viscera-related indexes. Multiple linear regression analysis showed that morphological indexes could explain only a small amount of variation for MFW (R2 = 0.20), indicating that the regression prediction was unsatisfactory, and direct measurement remained an effective approach. This study provided an accurate quantitative method for genetic improvement of body lipid traits in C. idella.
[1] |
农业农村部渔业渔政管理局, 全国水产技术推广总站, 中国水产学会. 中国渔业统计年鉴-2021[M]. 北京: 中国农业出版社, 2021.
Fishery Administration of the Ministry of Agriculture and Rural Affairs, National Fisheries Technology Extension Center, China Society of Fisheries. China fishery statistical yearbook-2021[M]. Beijing: China Agriculture Press, 2021 (in Chinese).
|
[2] |
姜鹏, 卢薛, 李胜杰, 等. 草鱼体脂性状的变异特征及相关性[J]. 水产学报, 2019, 43(7): 1626-1634.
Jiang P, Lu X, Li S J, et al. Phenotypic variability and correlation analysis of lipid traits in grass carp (Ctenopharyngodon idella)[J]. Journal of Fisheries of China, 2019, 43(7): 1626-1634 (in Chinese).
|
[3] |
董小林, 钱雪桥, 刘家寿, 等. 饲料蛋白质和小麦淀粉水平对中大规格草鱼生长性能及肝脏组织结构的影响[J]. 水生生物学报, 2019, 43(5): 983-991. doi: 10.7541/2019.117
Dong X L, Qian X Q, Liu J S, et al. Effects of dietary protein and wheat starch on growth and liver structure of grass carp, Ctenopharyngodon idella[J]. Acta Hydrobiologica Sinica, 2019, 43(5): 983-991 (in Chinese). doi: 10.7541/2019.117
|
[4] |
郭小泽, 梁旭方, 方刘, 等. 饲料中非蛋白能量源对草鱼血清生化指标和肝脏组织的影响[J]. 水生生物学报, 2014, 38(3): 582-587. doi: 10.7541/2014.82
Guo X Z, Liang X F, Fang L, et al. Effects of non-protein energy sources on serum biochemical indices and histology of liver in grass carp (Ctenopharyngodon idella)[J]. Acta Hydrobiologica Sinica, 2014, 38(3): 582-587 (in Chinese). doi: 10.7541/2014.82
|
[5] |
Guo X Z, Liang X F, Fang L, et al. Effects of dietary non-protein energy source levels on growth performance, body composition and lipid metabolism in herbivorous grass carp (Ctenopharyngodon idella Val. )[J]. Aquaculture Research, 2015, 46(5): 1197-1208. doi: 10.1111/are.12275
|
[6] |
陈拥军, 邹滔, 林仕梅, 等. 草鱼体组成的数学描述[J]. 水产学报, 2016, 40(4): 566-576.
Chen Y J, Zou T, Lin S M, et al. Quantitative description of body composition in grass carp (Ctenopharyngodon idella)[J]. Journal of Fisheries of China, 2016, 40(4): 566-576 (in Chinese).
|
[7] |
田丽霞, 刘永坚, 刘栋辉, 等. 葡萄糖和玉米淀粉对草鱼生长和肠系膜脂肪沉积的影响[J]. 水产学报, 2000, 24(5): 438-441.
Tian L X, Liu Y J, Liu D H, et al. Effects of glucose and corn starch on growth and the fat deposition in the mesentery of grass carp[J]. Journal of Fisheries of China, 2000, 24(5): 438-441 (in Chinese).
|
[8] |
Tian L X, Liu Y J, Hung S S O. Utilization of glucose and cornstarch by juvenile grass carp[J]. North American Journal of Aquaculture, 2004, 66(2): 141-145. doi: 10.1577/A03-040.1
|
[9] |
田丽霞, 刘永坚, 冯健, 等. 不同种类淀粉对草鱼生长、肠系膜脂肪沉积和鱼体组成的影响[J]. 水产学报, 2002, 26(3): 247-251.
Tian L X, Liu Y J, Feng J, et al. Effect of different types of starch on growth, the deposition of mesenteric fat and body composition of Ctenopharyngodon idellus[J]. Journal of Fisheries of China, 2002, 26(3): 247-251 (in Chinese).
|
[10] |
Dong G F, Zou Q, Wang H, et al. Conjugated linoleic acid differentially modulates growth, tissue lipid deposition, and gene expression involved in the lipid metabolism of grass carp[J]. Aquaculture, 2014, 432: 181-191. doi: 10.1016/j.aquaculture.2014.05.008
|
[11] |
程辉辉, 谢从新, 李大鹏, 等. 种青养鱼模式下的草鱼肌肉营养成分和品质特性[J]. 水产学报, 2016, 40(7): 1050-1059.
Cheng H H, Xie C X, Li D P, et al. The study of muscular nutritional components and fish quality of grass carp (Ctenopharyngodon idella) in ecological model of cultivating grass carp with grass[J]. Journal of Fisheries of China, 2016, 40(7): 1050-1059 (in Chinese).
|
[12] |
Mehlem A, Hagberg C E, Muhl L, et al. Imaging of neutral lipids by oil red O for analyzing the metabolic status in health and disease[J]. Nature Protocols, 2013, 8(6): 1149-1154. doi: 10.1038/nprot.2013.055
|
[13] |
王肖燕, 王金泉, 姚刚, 等. 脂肪组织冰冻切片油红O滴染法的建立[J]. 家畜生态学报, 2014, 35(8): 58-60,96. doi: 10.3969/j.issn.1673-1182.2014.08.012
Wang X Y, Wang J Q, Yao G, et al. Drop method of Oil red O staining on adipose tissue frozen section[J]. Acta Ecologiae Animalis Domastici, 2014, 35(8): 58-60,96 (in Chinese). doi: 10.3969/j.issn.1673-1182.2014.08.012
|
[14] |
Deutsch M J, Schriever S C, Roscher A A, et al. Digital image analysis approach for lipid droplet size quantitation of Oil Red O-stained cultured cells[J]. Analytical Biochemistry, 2014, 445: 87-89. doi: 10.1016/j.ab.2013.10.001
|
[15] |
Kinkel A D, Fernyhough M E, Helterline D L, et al. Oil red-O stains non-adipogenic cells: a precautionary note[J]. Cytotechnology, 2004, 46(1): 49-56. doi: 10.1007/s10616-004-3903-4
|
[16] |
Escorcia W, Ruter D L, Nhan J, et al. Quantification of lipid abundance and evaluation of lipid distribution in Caenorhabditis elegans by Nile red and oil red O staining[J]. Journal of Visualized Experiments, 2018, 133: e57352.
|
[17] |
Beattie J H, Duthie S J, Kwun I S, et al. Rapid quantification of aortic lesions in ApoE(−/−) mice[J]. Journal of Vascular Research, 2009, 46(4): 347-352. doi: 10.1159/000189795
|
[18] |
Ramírez-Zacarías J L, Castro-Muñozledo F, Kuri-Harcuch W. Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with oil red O[J]. Histochemistry, 1992, 97(6): 493-497. doi: 10.1007/BF00316069
|
[19] |
Wu J L, Zhang J L, Du X X, et al. Evaluation of the distribution of adipose tissues in fish using magnetic resonance imaging (MRI)[J]. Aquaculture, 2015, 448: 112-122. doi: 10.1016/j.aquaculture.2015.06.002
|