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Volume 48 Issue 11
Nov.  2024
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ZHOU Hangxian, TAO Mingwei, WEI Jie, ZHAO Jianhua, XU Qiyou. Effect of replacing glucose with soybean oligosaccharides on microbial community structure in biofloc system[J]. Journal of fisheries of china, 2024, 48(11): 119613. DOI: 10.11964/jfc.20230614058
Citation: ZHOU Hangxian, TAO Mingwei, WEI Jie, ZHAO Jianhua, XU Qiyou. Effect of replacing glucose with soybean oligosaccharides on microbial community structure in biofloc system[J]. Journal of fisheries of china, 2024, 48(11): 119613. DOI: 10.11964/jfc.20230614058
shu

Effect of replacing glucose with soybean oligosaccharides on microbial community structure in biofloc system

  • 1.

    School of Life Science, Huzhou University, Huzhou　313000, China

  • 2.

    National and Local Joint Engineering Laboratory of Aquatic Animal Breeding and Nutrition, Huzhou University, Huzhou　313000, China

  • 3.

    Zhejiang Provincial Key Laboratory for Conservation and Development of Aquatic Biological Resources, Huzhou University, Huzhou　313000, China

Funds: Zhejiang Province Agricultural Major Technology Cooperative Promotion Plan (2022XTTGSC01); Natural Science Foundation of Zhejiang Province ( LTGN23C190002 )
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    Graphical Abstract
    • Abstract

  • The study investigated the effects of soybean oligosaccharides (SBOS) on the microbial community structure within a biofloc system and identified the optimal concentration. The control group utilized glucose as carbon source, whereas experimental groups replaced it with 2.5%, 5.0%, 7.5%, and 10.0% SBOS, respectively. Ammonium chloride served as the nitrogen source with C/N ratio of 15, and Bacillus subtilis was introduced to establish the biofloc system. The results indicated that total nitrogen, ammonia nitrogen, nitrite nitrogen, and nitrate nitrogen concentrations in the water were reduced, with no significant differences observed among all groups (P>0.05). High-throughput 16S rRNA sequencing revealed that Proteobacteria was the dominant phylum at 14 days in both experimental and control groups. By 28 days, Proteobacteria and Bacteroidota proportions were similar. At the genus level, Bacillus abundance significantly increased in the SBOS-2.5 and SBOS-10.0 groups at 14 days (P<0.05), while Aeromonadaceae abundance significantly decreased (P<0.05). At day 28, Flavobacterium abundance significantly increased in the SBOS-2.5 and SBOS-10.0 groups compared to the control group (P<0.05). Additionally, Chryseobacterium abundance in all experimental groups was significantly lower than control group (P<0.05). Some bacteria may be crucial in the nitrification and denitrification of nitrogen in water, according to a correlation analysis between bacterial composition and environmental conditions. In conclusion, substituting glucose with 2.5% and 10.0% SBOS as the carbon source in bioflocs effectively enhanced the abundance of beneficial bacteria and reduced that of harmful bacteria, thus promoting the stability of bacterial communities in water. Considering experimental outcomes and application costs, a 2.5% SBOS replacement ratio for glucose is recommended. This study provides a foundation for the practical application of SBOS as a carbon source in the biofloc systems and offers insights for future research.

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