• ISSN 1000-0615
  • CN 31-1283/S
WANG Shentong, ZHANG Meng, SHEN Yubang, LI Jiale. Polymorphisms of the GH gene 3′ partial sequence and their associations with growth traits and muscle composition in juvenile grass carp (Ctenopharyngodon idella)[J]. Journal of fisheries of china, 2017, 41(9): 1329-1337. DOI: 10.11964/jfc.20160910546
Citation: WANG Shentong, ZHANG Meng, SHEN Yubang, LI Jiale. Polymorphisms of the GH gene 3′ partial sequence and their associations with growth traits and muscle composition in juvenile grass carp (Ctenopharyngodon idella)[J]. Journal of fisheries of china, 2017, 41(9): 1329-1337. DOI: 10.11964/jfc.20160910546

Polymorphisms of the GH gene 3′ partial sequence and their associations with growth traits and muscle composition in juvenile grass carp (Ctenopharyngodon idella)

Funds: 

China Agriculture Research System (CARS-46-04); Shanghai Engineering and Technology Center for Promoting Ability (16DZ2281200); University-Industry Cooperation Project of Tongwei (TW2014F003)

More Information
  • Corresponding author:

    LI Jiale. E-mail: jlli2009@126.com

  • Received Date: September 18, 2016
  • Revised Date: March 02, 2017
  • Available Online: November 26, 2017
  • Published Date: August 31, 2017
  • In order to comprehend whether the polymorphism of grass carp GH gene was associated with growth traits and muscle compositions, 156 individuals from Yangtze River were used in this study. Nine polymorphic loci were found in the GH gene 3′ partial sequence: G2825A、G2914T、T2966G、A3002T、T3022C、A3301G、C3463T、C3547T、C3620T, which were named SNP1-SNP9 respectively. Nine loci were not significantly deviated from Hardy-Weinberg equilibrium based on Chi-square test (P>0.05), and performed moderate polymorphism (0.25<PIC<0.5). The results of linkage disequilibrium showed that SNP3, SNP4, SNP5 were a set of completed linkage disequilibrium (r2=1) and SNP2, SNP7, SNP9 were a set of completed linkage disequilibrium (r2=1). Haplotype analysis for five loci in GH 3′partial sequence revealed 6 haplotypes, which Hap1(30.4%) had the greatest proportion, Hap6(4.8%) had the lowest proportion. A general linear model and multiple comparisons were used to analyze the correlation between those single nucleotide polymorphisms (SNPs) and grass carp traits. The results showed that the body weight, body length and fat content of individuals with the homozygous mutant genotype at SNP2 and SNP3 were significantly higher than wide-type and heterozygous mutant genotype. Similar results were found in diplotype combinations analysis. The body weight, body length and fat content of combination with heterozygous mutant genotype at SNP2 and SNP3 were significantly higher than other combinations. The results showed that the polymorphism of SNP2 and SNP3 in grass carp GH gene had a significant correlation with growth traits and muscle composition (P<0.05), and it might have utility as markers for practical breeding programs for growth traits and muscle composition in grass carp. Meanwhile, we have laid a foundation for further research on the functional verification of the relevant mutation sites.
  • [1]
    沈玉帮, 张俊彬, 李家乐. 草鱼种质资源研究进展[J]. 中国农学通报, 2011, 27(7): 369-373.

    Shen Y B, Zhang J B, Li J L. Advances in studies on genetic resources of grass carp [J]. Chinese Agricultural Science Bulletin, 2011, 27(7): 369-373 (in Chinese).
    [2]
    FAO. Fishery and aquaculture statistics [R]. FAO Fisheries & Aquaculture Report. Rome: FAO, 2014.
    [3]
    张晓萌, 马普, 王洪迪, 等. SNPs在水产动物中的研究进展[J]. 生物技术通报, 2013(8): 7-11.

    Zhang X M, Ma P, Wang H D, et al. Progresses of SNPs studies in aquaculture animals [J]. Biotechnology Bulletin, 2013(8): 7-11 (in Chinese).
    [4]
    谭新, 童金苟. SNPs及其在水产动物遗传学与育种学研究中的应用[J]. 水生生物学报, 2011, 35(2): 348-354.

    Tan X, Tong J G. SNPs and their applications in studies on genetics and breeding of aquaculture animals [J]. Acta Hydrobiologica Sinica, 2011, 35(2): 348-354 (in Chinese).
    [5]
    Chen T T, Marsh A, Shamblott M, et al. 6 structure and evolution of fish growth hormone and insulinlike growth factor genes [J]. Fish Physiology, 1994, 13: 179-209.
    [6]
    Zeng C, Liu X L, Wang W M, et al. Characterization of GHRs, IGFs and MSTNs, and analysis of their expression relationships in blunt snout bream, Megalobrama amblycephala [J]. Gene, 2014, 535(2): 239-249.
    [7]
    Cavari B, Funkenstein B, Chen T T, et al. Effect of growth hormone on the growth rate of the gilthead seabream (Sparus aurata), and use of different constructs for the production of transgenic fish [J]. Aquaculture, 1993, 111(1-4): 189-197.
    [8]
    赵君丽, 何峰, 温海深, 等. 雄性半滑舌鳎GH基因多态性与激素水平及生长性状相关性分析[J]. 中国海洋大学学报, 2014, 44(12): 35-40.

    Zhao J L, He F, Wen H S, et al. Correlation between the polymorphism of GH gene of male half-smooth tongue sole and their growth traits and hormone content [J]. Periodical of Ocean University of China, 2014, 44(12): 35-40 (in Chinese).
    [9]
    倪静, 尤锋, 刘思思, 等. 牙鲆GH基因第1外显子区微卫星标记与幼鱼生长性状的相关分析[J]. 动物学杂志, 2011, 46(5): 108-113.

    Ni J, You F, Liu S S, et al. Correlation analysis of microsatellite DNA Marker in the GH exon 1 region with growth traits of juvenile olive flounder Paralichthys olivaceus [J]. Chinese Journal of Zoology, 2011, 46(5): 108-113 (in Chinese).
    [10]
    刘峰, 鲁双庆, 刘臻, 等. 三种鳜鱼(Siniperca)生长激素基因内含子多态性的比较研究[J]. 海洋与湖沼, 2009, 40(4): 470-478.

    Liu F, Lu S Q, Liu Z, et al. The GH gene diversity among three Siniperca fish species[J]. Oceanologia Et Limnologia Sinica, 2009, 40(4): 470-478 (in Chinese).
    [11]
    莫赛军, 宋平, 罗大极, 等. 鲫鱼生长激素Ⅰ基因内含子2的多态性分析[J]. 遗传学报, 2004, 31(6): 582-590.

    Mo S J, Song P, Luo D J, et al. Polymorphism in intron 2 of crucian carp GH I gene [J]. Acta Genetica Sinica, 2004, 31(6): 582-590 (in Chinese).
    [12]
    刘小献, 白俊杰, 于凌云, 等. 草鱼载脂蛋白A-Ⅰ-1基因3'非编码区SNPs筛选及其与生长性状的关联分析[J]. 大连海洋大学学报, 2012, 27(1): 12-17.

    Liu X X, Bai J J, Yu L Y, et al. SNPs screening of 3'UTR in Apoprotein A-I-1 gene and its association with growth traits in grass carp [J]. Journal of Dalian Fisheries University, 2012, 27(1): 12-17 (in Chinese).
    [13]
    褚敏, 阎萍, 梁春年, 等. 大通牦牛MyoD1基因3'UTR SNPs多态性及其与生长性状相关性的研究[J]. 中国畜牧兽医, 2012, 39(2): 111-113.

    Chu M, Yan P, Liang C N, et al. Association between polymorphism of MyoD1 gene and growth traits in yak [J]. China Animal Husbandry & Veterinary Medicine, 2012, 39(2): 111-113 (in Chinese).
    [14]
    傅建军, 王荣泉, 沈玉帮, 等. 我国草鱼野生群体D-Loop序列遗传变异分析[J]. 水生生物学报, 2015, 39(2): 349-357.

    Fu J J, Wang R Q, Shen Y B, et al. Genetic variation analysis based on D-Loop sequences of wild populations of grass carp (Ctenopharyngodon idella) in China [J]. Acta Hydrobiologica Sinica, 2015, 39(2): 349-357 (in Chinese).
    [15]
    程汉良, 蒋飞, 彭永兴, 等. 野生与养殖草鱼肌肉营养成分比较分析[J]. 食品科学, 2013, 34(13): 266-270.

    Cheng H L, Jiang F, Peng Y X, et al. Comparison of nutrient composition of muscles of wild and farmed grass carp, Ctenopharyngodon idellus [J]. Food Science, 2013, 34(13): 266-270 (in Chinese).
    [16]
    Rivero E R C, Neves A C, Silva-Valenzuela M G, et al. Simple salting-out method for DNA extraction from formalin-fixed, paraffin-embedded tissues [J]. Pathology-Research and Practice, 2006, 202(7): 523-529.
    [17]
    Hall T A. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT [J]. Nucleic Acids Symposium Series, 1999, 41: 95-98.
    [18]
    Kalinowski S T, Taper M L, Marshall T C. Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment [J]. Molecular Ecology, 2007, 16(5): 1099-1106.
    [19]
    Coakes S J, Steed L. SPSS Version 14.0 for Windows: Analysis Without Anguish Using SPSS [M]. Milton: John Wiley & Sons, 2007.
    [20]
    Brookes A J. The essence of SNPs [J]. Gene, 1999, 234(2): 177-186.
    [21]
    Botstein D, White R L, Skolnick M, et al. Construction of a genetic linkage map in man using restriction fragment length polymorphisms [J]. American Journal of Human Genetics, 1980, 32(3): 314-331.
    [22]
    周靖晶. 中国人群年龄相关性黄斑变性与基因及环境因素的关联研究[D]. 武汉: 华中科技大学, 2013.

    Zhou J J. Association of genetic and environmental factors with age-related macular degeneration in a Chinese population [D]. Wuhan: Huazhong University of Science and Technology, 2013 (in Chinese).
    [23]
    Kruglyak L. Prospects for whole-genome linkage disequilibrium mapping of common disease genes [J]. Nature Genetics, 1999, 22(2): 139-144.
    [24]
    宋成义, 经荣斌. 畜禽GH基因多态性与生产性能相关研究进展[J]. 国外畜牧科技, 2000, 27(2): 25-27.

    Song C Y, Jing R B. Research on the relation between polymorphism in the stock and poultry GH gene and production performance [J]. Animal Science Abroad, 2000, 27(2): 25-27 (in Chinese).
    [25]
    倪静, 尤锋, 张培军, 等. 牙鲆GH基因外显子多态性与生长性状关系的初步研究[J]. 高技术通讯, 2006, 16(3): 307-312.

    Ning J, You F, Zhang P J, et al. Primary study on PCR-SSCP analysis of GH gene’s exon polymorphism in Paralichthys olivaceus and its association with growth traits among a hatchery stock [J]. Chinese High Technology Letters, 2006, 16(3): 307-312 (in Chinese).
    [26]
    Drysdale C M, Mcgraw D W, Stack C B, et al. Complex promoter and coding region β2-adrenergic receptor haplotypes alter receptor expression and predict in vivo responsiveness [J]. Proceedings of the National Academy of Sciences of the United States of America, 2000, 97(19): 10483-10488.
  • Cited by

    Periodical cited type(4)

    1. 薛雨,王秀利. 影响红鳍东方鲀生长性状的候选基因及其SNP的研究进展. 河北渔业. 2023(04): 33-36+46 .
    2. 殷艳慧,蒋万胜,潘晓赋,杨君兴. 水产养殖鱼类生长性状研究进展. 中国水产科学. 2020(04): 463-484 .
    3. 张猛,沈玉帮,徐晓雁,王荣泉,冷向军,李家乐. 草鱼7个插入/缺失型突变多态性及与幼鱼生长性状关联分析. 水产学报. 2019(08): 1706-1713 . 本站查看
    4. 薛宝宝,孟德龙,任泓妤,申奔龙,牛东红,李家乐,沈和定. 缢蛏新品种“申浙1号”ScHsc70基因SNPs筛查与耐高温性状的关联性分析. 水产学报. 2019(12): 2439-2448 . 本站查看

    Other cited types(3)

Catalog

    Article views (1288) PDF downloads (13) Cited by(7)
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return