• ISSN 1000-0615
  • CN 31-1283/S
Volume 45 Issue 2
Feb.  2021
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ZHAO Yueji, GUO Haipeng, ZHANG Demin. Effects of different culture patterns on the intestinal microbiota of Litopenaeus vannamei[J]. Journal of fisheries of china, 2021, 45(2): 221-234. DOI: 10.11964/jfc.20200312196
Citation: ZHAO Yueji, GUO Haipeng, ZHANG Demin. Effects of different culture patterns on the intestinal microbiota of Litopenaeus vannamei[J]. Journal of fisheries of china, 2021, 45(2): 221-234. DOI: 10.11964/jfc.20200312196
shu

Effects of different culture patterns on the intestinal microbiota of Litopenaeus vannamei

  • School of Marine Sciences, Ningbo University, Ningbo    315211, China

Funds: National Natural Science Foundation of China (31672658); Agricultural Major Project of Ningbo, China (2017C110001)
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  • Corresponding author:

    GUO Haipeng. E-mail: guohaipeng@nub.edu.cn

  • Received Date: March 17, 2020
  • Revised Date: May 25, 2020
  • Available Online: September 01, 2020
  • Published Date: January 31, 2021
    Graphical Abstract
    • Abstract
  • To explore the differences of intestinal microbiota for Litopenaeus vannamei in different culture patterns, bacterial diversity and bacterial community composition of L. vannamei from different culture patterns including freshwater pond (FWP), seawater pond (SP), high-intensive pond (HP) and biofloc technology pond (BFP) were studied by using the 16S rRNA gene amplicon sequencing technology in this study. The results showed that the α-diversity indexes of FWP and HP were significantly higher than those of SP and BFP. The relative abundances of intestinal microbiota compositions were significantly different among the four culture patterns. Proteobacteria had the maximal abundance in SP, BFP and HP culture patterns and contributed to the biggest differences for the four culture patterns. The abundance of Cyanobacteria in FWP culture pattern was far higher than that in other culture patterns. Vibrionaceae (Proteobacteria) and Mycoplasmataceae (Tenericutes) were the top two abundant families of core microbiota among the four culture patterns. In addition, the indicators of different culture patterns were different. The indicator group of intestinal bacteria in FWP culture pattern was Propionibacteriaceae, and it was Flavobacteriaceae in BFP culture pattern. Co-occurrence network analysis found that the interactions of intestinal microbiota in the HP culture pattern were higher than that in other three culture patterns, and the co-occurrence network of FWP was the most stable.
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