<i>IgM</i> expression and B lymphocyte antibacterial activity of grass carp (<i>Ctenopharyngodon idella</i>)

ZHANG Menglan; LU Zhijie; ZHAN Fanbin; LI Yanan; SHI Fei; ZHAO Lijuan; LIN Li; QIN Zhendong

doi:10.11964/jfc.20200612289

Volume 45 Issue 1

Jan. 2021

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Journal of fisheries of china > 2021 > 45(1): 115-124. > DOI: 10.11964/jfc.20200612289

ZHANG Menglan, LU Zhijie, ZHAN Fanbin, LI Yanan, SHI Fei, ZHAO Lijuan, LIN Li, QIN Zhendong. IgM expression and B lymphocyte antibacterial activity of grass carp (Ctenopharyngodon idella)[J]. Journal of fisheries of china, 2021, 45(1): 115-124. DOI: 10.11964/jfc.20200612289

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IgM expression and B lymphocyte antibacterial activity of grass carp (Ctenopharyngodon idella)

Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225

Funds: National Natural Science Foundation of China (3190240931872606, 3157265731602190, U1701233); Fund of Guangdong Provincial Bureau of Marine and Fisheries (GDME-2018C006, D21822202, A201512C0032015-115); Fund of Guangdong Department of Education (KA170500G, TK222001G, KA18058B3, KA1819604)

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Corresponding author:
LIN Li. E-mail: linli@zhku.edu.cn

QIN Zhendong. E-mail: qinzhendongsc@163.com
Received Date: June 06, 2020
Revised Date: August 18, 2020
Available Online: October 19, 2020
Published Date: December 31, 2020

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Abstract

Abstract

Grass carp (Ctenopharyngodon idella) is a unique aquaculture species in China, which is mainly distributed in the Yangtze River, Pearl River and Heilongjiang River, and its aquaculture output has long been the highest in freshwater aquaculture in China. With the increase of culture scale and density, the environment continues to deteriorate, resulting in frequent outbreaks of a variety of diseases. Therefore, it is necessary to study its disease resistance mechanism. B lymphocytes are the key cells of adaptive immune response, and can secrete a variety of immunoglobulins which play an important role in the immune response. Respiratory burst is an oxygen-dependent killing mechanism of immune cells when pathogenic microorganisms invade the body. Previous studies suggested that fish B lymphocytes can participate in non-specific immune process through phagocytosis. However, the study of the phagocytic activity of B lymphocytes in C. idella is still not well known. To elucidate the immune function of IgM, real-time fluorescence quantitative PCR (qRT-PCR) was used to detect the expression of IgM in embryonic development, the distribution of IgM in different tissues and the transcription of IgM stimulated by bacteria. The results showed that during embryonic development, the expression of IgM was not significantly changed from cleavage stage to pre-hatching stage, and it significantly increased after hatching stage; IgM was distributed in all tested tissues, with the highest expression in the head kidney, and its expression was significantly up-regulated after the stimulation of different bacteria. B lymphocytes were isolated and purified from the C. idella blood and verified by Giemsa staining, RT-PCR and indirect immunofluorescence. The results of bacterial phagocytosis test showed that B lymphocytes had phagocytic ability to Aeromonas hydrophila. Furthermore, we also found that the production of reactive oxygen species (ROS) and nitric oxide (NO) in grass carp B cells could be significantly up-regulated after being infected by microbes. Finally, the serum conditioning assay showed that the release of ROS from B lymphocytes could be enhanced through serum conditioning, however, there was no influence on NO production. Taken together, the results of this study showed that B lymphocytes of C. idella have the potential of phagocytic ability to bacteria and can directly participate in anti-bacterial immunity by producing non-specific immunity such as respiratory burst.
- Ctenopharyngodon idella,
- B lymphocytes,
- phagocytosis,
- IgM,
- respiratory burst

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IgM expression and B lymphocyte antibacterial activity of grass carp (Ctenopharyngodon idella)

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