Effects of aflatoxin B1 on growth performance and liver function of juvenile <i>Pelteobagrus fulvidraco</i>

ZHONG Lei; WANG Ziqin; WANG Jinlong; XIA Liqiu; CHEN Liyuan; ZHOU Jiancheng; HU Yi

doi:10.11964/jfc.20201212564

Volume 45 Issue 10

Oct. 2021

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Journal of fisheries of china > 2021 > 45(10): 1775-1786. > DOI: 10.11964/jfc.20201212564

ZHONG Lei, WANG Ziqin, WANG Jinlong, XIA Liqiu, CHEN Liyuan, ZHOU Jiancheng, HU Yi. Effects of aflatoxin B1 on growth performance and liver function of juvenile Pelteobagrus fulvidraco[J]. Journal of fisheries of china, 2021, 45(10): 1775-1786. DOI: 10.11964/jfc.20201212564

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Effects of aflatoxin B1 on growth performance and liver function of juvenile Pelteobagrus fulvidraco

1.
Hunan Engineering Research Center for Utilization of Characteristics Aquatic Resources, Hunan Agricultural University, Changsha 410128, China
2.
Collage of Life and Environmental Sciences, Hunan University of Arts and Science, Changde 415000, China
3.
Hunan Fisheries Science Institute, Changsha 410153, China
4.
College of Life Sciences, Hunan Normal University, Changsha 410081, China
5.
Jiangsu Aomai Bio-Technology Co.LTD, Nanjing 210000, China
6.
Wuhan DBN Aquaculture Technology Co. LTD, Wuhan 430090, China

Funds: Special technical system for freshwater fish industry with national characteristics (CARS-46); National key laboratory of freshwater fish development biology open fund (2020KF006); 2019 Science and technology cooperation project of DBN

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Corresponding author:
WANG Jinlong. E-mail: 124532076@qq.com

HU Yi. E-mail: huyi740322@163.com
Received Date: December 29, 2020
Revised Date: February 06, 2021
Available Online: June 15, 2021
Published Date: September 30, 2021

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Abstract

Abstract

An experiment was conducted to study the effect of aflatoxin B1 on growth performance, digestive enzyme activities in intestinal tract and liver function of juvenile Pelteobagrus fulvidraco. Four experimental diets were prepared to contain 0 (control), 50, 100, 200 μg/kg aflatoxin B1. Each diet was randomly assigned to triplicate cages of 50 fish with an initial average weight of (6.00±0.10) g for 8 weeks. The results showed that: ① There were no significant differences in survival rate, feed coefficient ratio and specific growth rates of juvenile Pelteobagrus fulvidraco among all groups. The activity of trypsin in the AFB1 group significantly increased compared with the control group. When the AFB1 concentration reached 100 and 200 μg/kg, the amylase and lipase activities significantly decreased; ② With increasing AFB1 concentration, the levels of aspartate aminotransferase(AST), alanine aminotransferase(ALT), glucose, triglyceride, total bile acid and total cholesterol contents in serum significantly increased, and the AST, ALT activities in the liver significantly decreased; ③With increasing AFB1 concentration, the catalase activity and malondiadehyde content in the liver significantly increased. The superoxide dismutase activity significantly increased when the AFB1 concentration reached 100 and 200 μg/kg; ④ With increasing AFB1 concentration, the expression levels of sod and il-1β in the liver were significantly up-regulated, the expression levels of cat, il-10 and il-8 in the liver were significantly up-regulated when the AFB1 concentration reached 200 μg/kg; ⑤ Histological observations showed that an increase in the concentration of AFB1 caused some liver cells to present slight atrophy, hepatocyte nucleus shifted, cell boundary blurred, liver cell vacuolation degre. The results showed that under the experimental conditions, there was no significant effect on the growth of juvenile Pelteobagrus fulvidraco with the concentration of AFB1≤200 μg/kg, but it could affect the digestion and absorption function of the intestines, cause oxidative stress and inflammatory reactions in the liver, and result in liver function damage with the concentration of AFB1≥50 μg/kg.
- Pelteobagrus fulvidraco,
- aflatoxin,
- growth performance,
- liver function

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[1]	伍皓茗, 苗玉涛. 黄颡鱼营养免疫研究进展[J]. 广东饲料, 2017, 26(2): 31-33. doi: 10.3969/j.issn.1005-8613.2017.02.010 Wu H M, Miao Y T. Advances in nutritional immunity of Pelteobagrus fulvidraco[J]. Guangdong Feed, 2017, 26(2): 31-33(in Chinese). doi: 10.3969/j.issn.1005-8613.2017.02.010
[2]	Santacroce M P, Conversano M C, Casalino E, et al. Aflatoxins in aquatic species: metabolism, toxicity and perspectives[J]. Reviews in Fish Biology and Fisheries, 2008, 18(1): 99-130.
[3]	Mahato D K, Lee K E, Kamle M, et al. Aflatoxins in food and feed: An overview on prevalence, detection and control strategies[J]. Frontiers in Microbiology, 2019, 10: 02266.
[4]	Nunes E M C G, Pereira M M G, Costa A P R, et al. Effects of aflatoxin B₁ on performance and health of tambaqui fingerlings (Colossoma macropomum)[J]. International Aquatic Research, 2019, 11(1): 73-83.
[5]	Deng S X, Tian L X, Liu F J, et al. Toxic effects and residue of aflatoxin B1 in tilapia (Oreochromis niloticus×O. aureus) during long-term dietary exposure[J]. Aquaculture, 2010, 307(3-4): 233-240.
[6]	Ayyat M S, Ayyat A M N, Al-Sagheer A A, et al. Effect of some safe feed additives on growth performance, blood biochemistry, and bioaccumulation of aflatoxin residues of Nile tilapia fed aflatoxin-B₁ contaminated diet[J]. Aquaculture, 2018, 495: 27-34.
[7]	Huang Y, Han D, Xiao X C, et al. Effect of dietary aflatoxin B₁ on growth, fecundity and tissue accumulation in gibel carp during the stage of gonad development[J]. Aquaculture, 2014, 428-429: 236-242.
[8]	Arana S, Alves V A F, Sabino M, et al. Immunohistochemical evidence for myofibroblast-like cells associated with liver injury induced by Aflatoxin B1 in rainbow trout (Oncorhynchus mykiss)[J]. Journal of Comparative Pathology, 2014, 150(2-3): 258-265.
[9]	Kowalska A, Walkiewicz K, Kozieł P, et al. Aflatoxins: characteristics and impact on human health[J]. Postepy Higieny I Medycyny Doswiadczalnej, 2017, 71: 315-327.
[10]	黄莹, 朱晓鸣, 韩冬, 等. 饲喂不同浓度黄曲霉毒素B₁饲料对异育银鲫成鱼的生长和毒素积累的影响[J]. 水生生物学报, 2012, 36(5): 817-825. Huang Y, Zhu X M, Han D, et al. Growth and aflatoxin B₁ accumulation of gibel carp adult fed with diets of different levels of aflatoxin B₁[J]. Acta Hydrobiologica Sinica, 2012, 36(5): 817-825(in Chinese).
[11]	黄莹, 韩金高, 朱晓鸣, 等. 饲喂不同浓度黄曲霉毒素B₁饲料对花鳗鲡幼鱼生长、抗氧化能力和毒素积累的影响[J]. 水生生物学报, 2021, 45(3): 566-572. Huang Y, Han J G, Zhu X M, et al. Effects of dietary aflatoxin B₁ on growth, antioxidant capacity and tissue accumulation of juvenile marbled eel (Anguilla marmorata)[J]. Acta Hydrobiologica Sinica, 2021, 45(3): 566-572(in Chinese).
[12]	Raghavan P R, Zhu X, Lei W, et al. Low levels of aflatoxin B₁ could cause mortalities in juvenile hybrid sturgeon, Acipenser ruthenus ♂×A. baeri♀[J]. Aquaculture Nutrition, 2011, 17(2): e39-e47.
[13]	Zeng S L, Long W Q, Tian L X, et al. Effects of dietary aflatoxin B₁ on growth performance, body composition, haematological parameters and histopathology of juvenile Pacific white shrimp (Litopenaeus vannamei)[J]. Aquaculture Nutrition, 2016, 22(5): 1152-1159.
[14]	Yu Y Y, Niu J, Yin P, et al. Detoxification and immunoprotection of Zn(II)-curcumin in juvenile Pacific white shrimp (Litopenaeus vannamei) feed with aflatoxin B1[J]. Fish & Shellfish Immunology, 2018, 80: 480-486.
[15]	Gonçalves R A, Cam T D, Tri N N, et al. Aflatoxin B₁ (AFB₁) reduces growth performance, physiological response, and disease resistance in Tra catfish (Pangasius hypophthalmus)[J]. Aquaculture International, 2018, 26(3): 921-936.
[16]	黄莹, 姚远, 朱晓鸣, 等. 饲喂不同浓度黄曲霉毒素B₁饲料对草鱼幼鱼生长和毒素积累的影响[J]. 水生生物学报, 2019, 43(4): 723-730. doi: 10.7541/2019.085 Huang Y, Yao Y, Zhu X M, et al. Effects of aflatoxin B₁ on growth and tissue accumulation of juvenile grass carp (Ctenopharyngodon idellus)[J]. Acta Hydrobiologica Sinica, 2019, 43(4): 723-730(in Chinese). doi: 10.7541/2019.085
[17]	El-Sayed Y S, Khalil R H. Toxicity, biochemical effects and residue of aflatoxin B₁ in marine water-reared sea bass (Dicentrarchus labrax L.)[J]. Food and Chemical Toxicology, 2009, 47(7): 1606-1609.
[18]	Fan Y, Liu L T, Zhao L H, et al. Influence of Bacillus subtilis ANSB060 on growth, digestive enzyme and aflatoxin residue in Yellow River carp fed diets contaminated with aflatoxin B₁[J]. Food and Chemical Toxicology, 2018, 113: 108-114.
[19]	齐灿灿, 王宝杰, 刘梅, 等. 黄曲霉毒素B1(AFB1)的短期投喂对凡纳滨对虾肠道黏膜屏障的影响[J]. 水产学报, 2017, 41(12): 1936-1945. Qi C C, Wang B J, Liu M, et al. Effects of short term addition of aflatoxin B1(AFB1) on the intestinal mucosal barrier of Litopenaeus vannamei[J]. Journal of Fisheries of China, 2017, 41(12): 1936-1945(in Chinese).
[20]	Andleeb S, Ashraf M, Hafeez-Ur-Rehman M, et al. Effect of aflatoxin B₁-contaminated feed on growth and vital organs of advance fry of Catla catla[J]. Journal of Animal and Plant Sciences, 2015, 25(3): 816-825.
[21]	毕小娟, 陈代文, 余冰, 等. 黄曲霉毒素B₁对断奶仔猪生长性能、肝脏组织及肠道健康的影响[J]. 动物营养学报, 2018, 30(8): 3276-3284. doi: 10.3969/j.issn.1006-267x.2018.08.047 Bi X J, Chen D W, Yu B, et al. Effects of aflatoxin B₁ on growth performance, liver tissue and intestine health of weaned piglets[J]. Chinese Journal of Animal Nutrition, 2018, 30(8): 3276-3284(in Chinese). doi: 10.3969/j.issn.1006-267x.2018.08.047
[22]	冯光德. 自然霉变玉米对肉鸭生产性能和消化生理的影响及机制研究[D]. 雅安: 四川农业大学, 2011. Feng G D. Effects of corn naturally contaminated with mycotoxins on performance and digestive physiology of ducks and its mechanism[D]. Yaan: Sichuan Agricultural University, 2011 (in Chinese).
[23]	Han X Y, Huang Q C, Li W F, et al. Changes in growth performance, digestive enzyme activities and nutrient digestibility of cherry valley ducks in response to aflatoxin B₁ levels[J]. Livestock Science, 2008, 119(1-3): 216-220.
[24]	旦增曲珍, 廖雪莲, 侯晨姝, 等. 胰蛋白酶在脓毒症大鼠模型血清及组织中的表达及意义[J]. 中华内科杂志, 2018, 57(7): 505-510. doi: 10.3760/cma.j.issn.0578-1426.2018.07.007 Dan Z Q Z, Liao X L, Hou C Z, et al. The expression of trypsin in serum and vital organs of septic rats[J]. Chinese Journal of Internal Medicine, 2018, 57(7): 505-510(in Chinese). doi: 10.3760/cma.j.issn.0578-1426.2018.07.007
[25]	何杰, 吴代武, 叶元土, 等. 饲料组胺水平对黄颡鱼生长性能、血清生化指标和胃肠道黏膜结构的影响[J]. 动物营养学报, 2018, 30(7): 2581-2593. doi: 10.3969/j.issn.1006-267x.2018.07.018 He J, Wu D W, Ye Y T, et al. Effects of dietary histamine level on growth performance, serum biochemical indexes and gastrointestinal mucosa structure of Yellow Catfish (Pelteobagrus fulvidraco)[J]. Chinese Journal of Animal Nutrition, 2018, 30(7): 2581-2593(in Chinese). doi: 10.3969/j.issn.1006-267x.2018.07.018
[26]	Yan Q, Xie S, Zhu X, et al. Dietary methionine requirement for juvenile rockfish, Sebastes schlegeli[J]. Aquaculture Nutrition, 2007, 13(3): 163-169.
[27]	Han D, Xie S, Zhu X, et al. Growth and hepatopancreas performances of gibel carp fed diets containing low levels of aflatoxin B₁[J]. Aquaculture Nutrition, 2010, 16(4): 335-342.
[28]	王静. 饲料中黄曲霉毒素B₁对凡纳滨对虾生长、生化指标及肝胰腺显微结构的影响[D]. 保定: 河北农业大学, 2014. Wang J. Toxic effects of aflatoxin B₁ on growth performance, biochemical and hepatopancreas microstructure of Litopenaeus vannamei[D]. Baoding: Agricultural University of Hebei, 2014 (in Chinese).
[29]	石勇, 胡毅, 刘艳莉, 等. 血根碱对LPS诱导后黄鳝免疫应激及肠道炎症相关基因表达的影响[J]. 中国水产科学, 2020, 27(1): 125-136. Shi Y, Hu Y, Liu Y L, et al. Effects of sanguinarine on immune and intestinal inflammation related to gene expression in rice field eels (Monopterus albus) induced by LPS[J]. Journal of Fishery Sciences of China, 2020, 27(1): 125-136(in Chinese).
[30]	张媛媛, 宋理平, 胡斌, 等. 饲料中添加姜黄素对尼罗罗非鱼幼鱼生长和四氯化碳诱导肝损伤的影响[J]. 中国水产科学, 2018, 25(6): 1271-1280. Zhang Y Y, Song L P, Hu B, et al. Effect of curcumin on growth performance and protective effect of liver injury induced by carbon tetrachloride in Oreochromis niloticus[J]. Journal of Fishery Sciences of China, 2018, 25(6): 1271-1280(in Chinese).
[31]	Zhao W, Wang L, Liu M, et al. Transcriptome, antioxidant enzyme activity and histopathology analysis of hepatopancreas from the white shrimp Litopenaeus vannamei fed with aflatoxin B1(AFB1)[J]. Developmental & Comparative Immunology, 2017, 74: 69-81.
[32]	周小新. 牛磺酸对黄曲霉毒素B1中毒大鼠脾脏功能影响的研究[D]. 沈阳: 沈阳农业大学, 2019. Zhou X X. Effect of taurine on spleen function of aflatoxin B1 poisoned rats[D]. Shenyang: Shenyang Agricultural University, 2019 (in Chinese).
[33]	Lu C, Ling F, Ji J, et al. Expression of immune-related genes in goldfish gills induced by Dactylogyrus intermedius infections[J]. Fish & Shellfish Immunology, 2013, 34(1): 372-377.
[34]	Moore K W, de Waal Malefy R, Coffman R L, et al. Interleukin-10 and the interleukin-10 receptor[J]. Annual Review of Immunology, 2001, 19(1): 683-765.

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Effects of aflatoxin B1 on growth performance and liver function of juvenile Pelteobagrus fulvidraco

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