Effects of stocking density on growth, antioxidant enzyme activity and related gene expression of juvenile Siberian hybrid sturgeon <i>Acipenser baerii</i> (♀)×<i>A. schrenckii</i> (♂)

ZHAO Daxian; CHENG Chao; XIAO Min; ZHU Minjie; LI Linjie; MEI Feng; ZHA Yunfei; JIAN Shaoqing

doi:10.11964/jfc.20210512834

Volume 46 Issue 9

Sep. 2022

Turn off MathJax

Article Contents

Abstract

References

Journal of fisheries of china > 2022 > 46(9): 1582-1592. > DOI: 10.11964/jfc.20210512834

ZHAO Daxian, CHENG Chao, XIAO Min, ZHU Minjie, LI Linjie, MEI Feng, ZHA Yunfei, JIAN Shaoqing. Effects of stocking density on growth, antioxidant enzyme activity and related gene expression of juvenile Siberian hybrid sturgeon [Acipenser baerii (♀)×A. schrenckii (♂)][J]. Journal of fisheries of china, 2022, 46(9): 1582-1592. DOI: 10.11964/jfc.20210512834

Citation:

PDF (2267 KB)

Effects of stocking density on growth, antioxidant enzyme activity and related gene expression of juvenile Siberian hybrid sturgeon [Acipenser baerii (♀)×A. schrenckii (♂)]

1.
Jiangxi Provincal Key Laboratory of Aquatic Animal Resources and Utilization, School of Life Sciences, Nanchang University, Nanchang 330031, China
2.
Ji' an City Jinggangshan Mountains Agricalture Science and Technology Park, Ji' an 343000, China
3.
Jinggangshan Muyun Special Animal Breeding Co., Ltd., Ji' an 343000, China

Funds: National Key Research and Development Program (2018YFD0901704); Jiangxi Province Modern Agricultural Industrial Technology System Special Project (JXARS-03)

More Information

Corresponding author:
JIAN Shaoqing. E-mail: jianshaoqing@ncu.edu.cn
Received Date: May 10, 2021
Revised Date: November 04, 2021
Available Online: August 01, 2022
Published Date: August 31, 2022

Graphical Abstract

Abstract

Abstract

In order to study the effects of aquaculture density on the growth performance of juvenile Siberian hybrid sturgeons [Acipenser baerii (♀)×A. schrenckii (♂)] in Jinggangshan region, this study took juvenile Siberian hybrid sturgeons with an initial body weight of (7.89±0.45) g as the research object. Three density groups [low density group (1.16 kg/m³), medium density group (1.74 kg/m³) and high density group (2.32 kg/m³)] were set for 90 days of production test. Through the comparative analysis of different density groups of Siberian hybrid sturgeon growth indexes and body composition content, antioxidant enzyme activity and related gene expression levels, it was found that low density group of larval final body weight, weight gain rate and specific growth rate of low density group was significantly higher than those of medium density and high density group (P<0.05), the density experiment fish survival rate had no significant difference; water content and ash content increased with the increase of culture density, and there were significant differences, but crude fat and crude protein did not show significant differences. The activities of superoxide dismutase (SOD) and glutathione sulfur transferase (GSH-ST) in liver in low and medium density groups were significantly higher than those in high density group at the end of experiment (P<0.05). With the increase of breeding density, the content of MDA in liver of juvenile fish increased continuously, and the activity of catalase (CAT) decreased. There was no significant difference between MDA and CAT during the trial. The results also showed that the expression levels of growth-related growth hormone receptor gene (GHR), insulin-like growth factor-1 gene (IGF-1) and insulin-like growth factor-2 gene (IGF-2) were significantly decreased in the high-density group (P<0.05). The expression level of stress-related heat shock protein 70 gene (HSP70) in high density group was different after 45 days (P<0.05), and after 90 days, high density group was significantly higher than low density group (P<0.05).The expression level of heat shock protein 90 gene (HSP90) in low density group was significantly lower than those in medium and high density groups on day 30 (P<0.05). At the end of the experiment, there were significant differences among the three density groups (P<0.05). The results showed that the juvenile Siberian hybrid sturgeon showed good growth performance under medium and low density conditions. Therefore, it is suggested to control the breeding density of juvenile Siberian hybrid sturgeon in Jinggangshan Mountains region between 1.16 and 1.74 kg/m³.
- Acipenser baerii (♀)×A. schrenckii (♂),
- rearing density,
- growth,
- antioxidant enzymes,
- gene expression,
- Jinggangshan Mountains

FullText(HTML)

References (47)

References

[1]	齐茜, 刘浩浩, 李忠华, 等. 施氏鲟、西伯利亚鲟及其杂交后代的繁殖性能、生长性能及抗病性能比较[J]. 江西农业大学学报, 2017, 39(2): 376-383. doi: 10.13836/j.jjau.2017049 Qi Q, Liu H H, Li Z H, et al. Comparison of reproductive performance, growth performance and disease resistance performance among Acipenser schrencki, Acipenser baeri and their hybrid offsprings[J]. Acta Agriculturae Universitatis Jiangxiensis, 2017, 39(2): 376-383 (in Chinese). doi: 10.13836/j.jjau.2017049
[2]	Jodun W A, Millard M J, Mohler J. The effect of rearing density on growth, survival, and feed conversion of juvenile Atlantic sturgeon[J]. North American Journal of Aquaculture, 2002, 64(1): 10-15. doi: 10.1577/1548-8454(2002)064<0010:TEORDO>2.0.CO;2
[3]	Aidos L, Vasconi M, Abbate F, et al. Effects of stocking density on reared Siberian sturgeon (Acipenser baerii) larval growth, muscle development and fatty acids composition in a recirculating aquaculture system[J]. Aquaculture Research, 2019, 50(2): 588-598. doi: 10.1111/are.13936
[4]	Wunderink Y S, Engels S, Halm S, et al. Chronic and acute stress responses in Senegalese sole (Solea senegalensis): the involvement of cortisol, CRH and CRH-BP[J]. General and Comparative Endocrinology, 2011, 171(2): 203-210. doi: 10.1016/j.ygcen.2011.01.010
[5]	Ellis T, North B, Scott A P, et al. The relationships between stocking density and welfare in farmed rainbow trout[J]. Journal of Fish Biology, 2002, 61(3): 493-531. doi: 10.1111/j.1095-8649.2002.tb00893.x
[6]	Fairchild E A, Howell W H. Optimal stocking density for juvenile winter flounder Pseudopleuronectes americanus[J]. Journal of the World Aquaculture Society, 2001, 32(3): 300-308. doi: 10.1111/j.1749-7345.2001.tb00453.x
[7]	Wuertz S, Lutz I, Gessner J, et al. The influence of rearing density as environmental stressor on cortisol response of shortnose sturgeon (Acipenser brevirostrum)[J]. Journal of Applied Ichthyology, 2006, 22(S1): 269-273. doi: 10.1111/j.1439-0426.2007.00966.x
[8]	倪金金, 王裕玉, 徐钢春, 等. 养殖密度对池塘循环水养殖大口黑鲈生长、生理指标和GH、IGF-I基因表达的影响[J]. 大连海洋大学学报, 2020, 35(6): 805-813. Ni J J, Wang Y Y, Xu G C, et al. Effects of stocking density on growth, physiological indices and expression of GH and IGF-I genes of largemouth bass Micropterus salmoides in in-pond raceway culture systems[J]. Journal of Dalian Fisheries University, 2020, 35(6): 805-813 (in Chinese).
[9]	施培松. 匙吻鲟和鳙的生长、肌肉品质比较及FAS基因克隆与表达[D]. 武汉: 华中农业大学, 2013. Shi P S. Cloning and expression of fatty acid synthase gene and comparison on growth, muscle quality of Polyodon spathula and Aristichthys nobilis[D]. Wuhan: Huazhong Agricultural University, 2013 (in Chinese).
[10]	施志仪, 程千千, 宋佳坤. 西伯利亚鲟β-actin基因cDNA全长克隆、序列分析及其作为内参基因的应用研究[J]. 中国水产科学, 2010, 17(6): 1173-1182. Shi Z Y, Cheng Q Q, Song J K. Cloning and sequence analysis of Siberian sturgeon full-length β-actin gene cDNA and application of genes as a internal reference[J]. Journal of Fishery Sciences of China, 2010, 17(6): 1173-1182 (in Chinese).
[11]	武梦斌, 叶欢, 岳华梅, 等. 达氏鲟实时荧光定量PCR内参基因的筛选[J]. 中国水产科学, 2020, 27(7): 759-770. Wu M B, Ye H, Yue H M, et al. Identification of reference genes for qRT-PCR in Dabry’s sturgeon, Acipenser dabryanus[J]. Journal of Fishery Sciences of China, 2020, 27(7): 759-770 (in Chinese).
[12]	Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2^{−ΔΔC _T} method[J]. Methods, 2001, 25(4): 402-408. doi: 10.1006/meth.2001.1262
[13]	Ni M, Wen H S, Li J F, et al. The physiological performance and immune responses of juvenile Amur sturgeon (Acipenser schrenckii) to stocking density and hypoxia stress[J]. Fish & Shellfish Immunology, 2014, 36(2): 325-335. doi: 10.1016/j.fsi.2013.12.002
[14]	步艳, 李吉方, 温海深, 等. 放养密度对流水池塘养殖大杂交鲟生长及摄食的影响[J]. 海洋湖沼通报, 2013(4): 43-48. Bu Y, LI J F, Wen H S, et al. Effect of stocking density on growth and feeding of hybrid sturgeon[J]. Transactions of Oceanology and Limnology, 2013(4): 43-48 (in Chinese).
[15]	Wafa A H, Pierre D, Daniel B. Modelling growth and food intake rhythms of brook trout Salvelinus fontinalis under the effects of density and ration[J]. Ecological Modelling, 2004, 175(4): 385-394. doi: 10.1016/j.ecolmodel.2003.10.024
[16]	庄平, 李大鹏, 王明学, 等. 养殖密度对史氏鲟稚鱼生长的影响[J]. 应用生态学报, 2002, 13(6): 735-738. doi: 10.3321/j.issn:1001-9332.2002.06.022 Zhuang P, Li D P, Wang M X, et al. Effect of stocking density on growth of juvenile Acipenser schrenckii[J]. Chinese Journal of Applied Ecology, 2002, 13(6): 735-738 (in Chinese). doi: 10.3321/j.issn:1001-9332.2002.06.022
[17]	Garcia F, Romera D M, Gozi K S, et al. Stocking density of Nile tilapia in cages placed in a hydroelectric reservoir[J]. Aquaculture, 2013, 410-411: 51-56. doi: 10.1016/j.aquaculture.2013.06.010
[18]	Karnatak G, Das B K, Puthiyottil M, et al. Impact of stocking density on growth, feed utilization and survival of cage reared minor carp, Labeo bata (Hamilton, 1822) in Maithon reservoir, India[J]. Aquaculture, 2020, 532: 736078. doi: 10.1016/j.aquaculture.2020.736078
[19]	Oké V, Goosen N J. The effect of stocking density on profitability of African catfish (Clarias gariepinus) culture in extensive pond systems[J]. Aquaculture, 2019, 507: 385-392. doi: 10.1016/j.aquaculture.2019.04.043
[20]	Niazi E H N, Imanpoor M, Taghizadeh V, et al. Effects of density stress on growth indices and survival rate of goldfish (Carassius auratus)[J]. Global Veterinaria, 2013, 10(3): 365-371. doi: 10.5829/idosi.gv.2013.10.3.72112
[21]	张奇. 圈养模式下养殖密度对大口黑鲈生长、生理指标以及肌肉品质影响的研究[D]. 武汉: 华中农业大学, 2020. Zhang Q. Study on the effects of stocking density on growth, physiological indexes and muscle quality of Micropterus salmoides[D]. Wuhan: Huazhong Agricultural University, 2020 (in Chinese).
[22]	Breck J E. Body composition in fishes: body size matters[J]. Aquaculture, 2014, 433: 40-49. doi: 10.1016/j.aquaculture.2014.05.049
[23]	步艳, 李吉方, 温海深, 等. 养殖密度对大杂交鲟幼鱼体组份和血液生化指标的影响[J]. 海洋湖沼通报, 2014(3): 69-74. doi: 10.13984/j.cnki.cn37-1141.2014.03.010 Bu Y, Li J F, Wen H S, et al. Effect of stocking density on body composition and blood biochemistry of hybrid sturgeon[J]. Transactions of Oceanology and Limnology, 2014(3): 69-74 (in Chinese). doi: 10.13984/j.cnki.cn37-1141.2014.03.010
[24]	Dennis T E, Bulger A J. Condition factor and whole-body sodium concentrations in a freshwater fish: evidence for acidification stress and possible ionoregulatory over-compensation[J]. Water, Air, and Soil Pollution, 1995, 85(2): 377-382. doi: 10.1007/BF00476858
[25]	张墨, 李吉方, 温海深, 等. 放养密度对大杂交鲟生长性能的影响及生理应答机制[J]. 海洋科学, 2016, 40(8): 35-41. doi: 10.11759//hykx20150512001 Zhang M, Li J F, Wen H S, et al. Effect of stocking density on growth performance and mechanism of physiological response in hybrid sturgeon[J]. Marine Sciences, 2016, 40(8): 35-41 (in Chinese). doi: 10.11759//hykx20150512001
[26]	白庆利, 于洪贤, 张玉勇, 等. 养殖密度对哲罗鱼稚鱼生长和存活的影响[J]. 东北林业大学学报, 2009, 37(2): 63-64,70. doi: 10.3969/j.issn.1000-5382.2009.02.022 Bai Q L, Yu H X, Zhang Y Y, et al. Effect of stocking density on growth and survival of juvenile Hucho taimen during domestication[J]. Journal of Northeast Forestry University, 2009, 37(2): 63-64,70 (in Chinese). doi: 10.3969/j.issn.1000-5382.2009.02.022
[27]	张玉勇, 徐革锋, 金舒博, 等. 养殖密度对饵料驯化期细鳞鱼稚鱼生长的影响[J]. 上海水产大学学报, 2008, 17(3): 321-326. Zhang Y Y, Xu G F, Jin S B, et al. Influences of stocking density of domestication on growth of juvenile Brachymastax lenok[J]. Journal of Shanghai Fisheries University, 2008, 17(3): 321-326 (in Chinese).
[28]	Mylonas C C, Fostier A, Zanuy S. Broodstock management and hormonal manipulations of fish reproduction[J]. General and Comparative Endocrinology, 2010, 165(3): 516-534. doi: 10.1016/j.ygcen.2009.03.007
[29]	Wagner E J, Intelmann S S, Routledge M D. The effects of fry rearing density on hatchery performance, fin condition, and agonistic behavior of rainbow trout Oncorhynchus mykiss fry[J]. Journal of the World Aquaculture Society, 1996, 27(3): 264-274. doi: 10.1111/j.1749-7345.1996.tb00608.x
[30]	Senso L, Suárez M D, Ruiz-Cara T, et al. On the possible effects of harvesting season and chilled storage on the fatty acid profile of the fillet of farmed gilthead sea bream (Sparus aurata)[J]. Food Chemistry, 2007, 101(1): 298-307. doi: 10.1016/j.foodchem.2006.01.036
[31]	Braun N, de Lima R L, Baldisserotto B, et al. Growth, biochemical and physiological responses of Salminus brasiliensis with different stocking densities and handling[J]. Aquaculture, 2010, 301(1-4): 22-30. doi: 10.1016/j.aquaculture.2010.01.022
[32]	方允中, 杨胜, 伍国耀. 自由基、抗氧化剂、营养素与健康的关系[J]. 营养学报, 2003, 25(4): 337-343. doi: 10.3321/j.issn:0512-7955.2003.04.001 Fang Y Z, Yang S, Wu G Y. Free radicals, antioxidants, and nutrients in relation to health[J]. Acta Nutrimenta Sinica, 2003, 25(4): 337-343 (in Chinese). doi: 10.3321/j.issn:0512-7955.2003.04.001
[33]	Tauler P, Aguiló A, Gimeno I, et al. Influence of vitamin C diet supplementation on endogenous antioxidant defences during exhaustive exercise[J]. Pflügers Archiv - European Journal of Physiology, 2003, 446(6): 658-664. doi: 10.1007/s00424-003-1112-1
[34]	宋志飞. 养殖密度对流水养殖系统中俄罗斯鲟幼鱼生长、血液生理生化以及非特异性免疫的影响[D]. 青岛: 中国海洋大学, 2015. Song Z F. The influence of stocking density on the growth performance, physiological and biochemical indices and nonspecific immunity of juvenile Russian sturgeon in flowing water cultivation[D]. Qingdao: Ocean University of China, 2015 (in Chinese).
[35]	Long L N, Zhang H G, Ni Q, et al. Effects of stocking density on growth, stress, and immune responses of juvenile Chinese sturgeon (Acipenser sinensis) in a recirculating aquaculture system[J]. Comparative Biochemistry and Physiology-Part C: Toxicology & Pharmacology, 2019, 219: 25-34. doi: 10.1016/j.cbpc.2019.02.002
[36]	宋文华. 养殖密度和温度对草鱼(Ctenopharyngodon idellus)生长和生理生化指标的影响[D]. 青岛: 中国海洋大学, 2012. Song W H. The effects of stocking density and water temperature on growth and physiological parameters of grass carp[D]. Qingdao: Ocean University of China, 2012 (in Chinese).
[37]	王裕玉, 徐跑, 聂志娟, 等. 池塘工程化循环水养殖模式下养殖密度对吉富罗非鱼生长及生理指标的影响[J]. 长江大学学报(自然科学版), 2019, 16(5): 78-84. Wang Y Y, Xu P, Nie Z J, et al. Effects of stocking densities on growth, serum biochemical parameters and antioxidant status of juvenile GIFT tilapia (Oreochromis niloticus) reared in in-pond raceway recirculating culture system[J]. Journal of Yangtze University (Natural Science Edition), 2019, 16(5): 78-84 (in Chinese).
[38]	Liu B L, Jia R, Han C, et al. Effects of stocking density on antioxidant status, metabolism and immune response in juvenile turbot (Scophthalmus maximus)[J]. Comparative Biochemistry and Physiology-Part C: Toxicology & Pharmacology, 2016, 190: 1-8. doi: 10.1016/j.cbpc.2016.07.007
[39]	Sahin K, Yazlak H, Orhan C, et al. The effect of lycopene on antioxidant status in rainbow trout (Oncorhynchus mykiss) reared under high stocking density[J]. Aquaculture, 2014, 418-419: 132-138. doi: 10.1016/j.aquaculture.2013.10.009
[40]	倪金金, 王裕玉, 徐钢春, 等. 养殖密度对池塘工程化循环水养殖大口黑鲈抗氧化力、组织结构及应激基因表达的影响[J]. 中国水产科学, 2020, 27(6): 660-670. Ni J J, Wang Y Y, Xu G C, et al. Effects of stocking density on the antioxidant status, tissue structure, and HSP70 and Cu/Zn-SOD expression in largemouth bass (Micropterus salmoides) in an in-pond raceway culture system[J]. Journal of Fishery Sciences of China, 2020, 27(6): 660-670 (in Chinese).
[41]	李洁, 唐夏, 张灵燕, 等. 溶解氧水平对褐牙鲆幼鱼能量代谢和氧化应激的影响研究[J]. 广西科学院学报, 2015, 31(1): 22-27. doi: 10.3969/j.issn.1002-7378.2015.01.004 Li J, Tang X, Zhang L Y, et al. Study on the energy metabolism and oxidative stress of juvenile brown flounder, Paralichthys olivaceus in dissolved oxygen content descending water[J]. Journal of Guangxi Academy of Sciences, 2015, 31(1): 22-27 (in Chinese). doi: 10.3969/j.issn.1002-7378.2015.01.004
[42]	Reindl K M, Sheridan M A. Peripheral regulation of the growth hormone-insulin-like growth factor system in fish and other vertebrates[J]. Comparative Biochemistry and Physiology-Part A: Molecular & Integrative Physiology, 2012, 163(3-4): 231-245. doi: 10.1016/j.cbpa.2012.08.003
[43]	Allard J B, Duan C M. Comparative endocrinology of aging and longevity regulation[J]. Frontiers in Endocrinology, 2011, 2: 75. doi: 10.3389/FENDO.2011.00075
[44]	Ren Y Y, Wen H S, Li Y, et al. Stocking density affects the growth performance and metabolism of Amur sturgeon by regulating expression of genes in the GH/IGF axis[J]. Journal of Oceanology and Limnology, 2018, 36(3): 956-972. doi: 10.1007/s00343-018-7018-8
[45]	de las Heras V, Martos-Sitcha J A, Yúfera M, et al. Influence of stocking density on growth, metabolism and stress of thick-lipped grey mullet (Chelon labrosus) juveniles[J]. Aquaculture, 2015, 448: 29-37. doi: 10.1016/j.aquaculture.2015.05.033
[46]	Viant M R, Werner I, Rosenblum E S, et al. Correlation between heat-shock protein induction and reduced metabolic condition in juvenile steelhead trout (Oncorhynchus mykiss) chronically exposed to elevated temperature[J]. Fish Physiology and Biochemistry, 2003, 29(2): 159-171. doi: 10.1023/B:FISH.0000035938.92027.81
[47]	Zahedi S, Akbarzadeh A, Mehrzad J, et al. Effect of stocking density on growth performance, plasma biochemistry and muscle gene expression in rainbow trout (Oncorhynchus mykiss)[J]. Aquaculture, 2019, 498: 271-278. doi: 10.1016/j.aquaculture.2018.07.044

Cited By

Get Citation

PDF in Chinese

XML

Article views (668) PDF downloads (27)

Effects of stocking density on growth, antioxidant enzyme activity and related gene expression of juvenile Siberian hybrid sturgeon [Acipenser baerii (♀)×A. schrenckii (♂)]

Abstract

References

Catalog

Related

Effects of stocking density on growth, antioxidant enzyme activity and related gene expression of juvenile Siberian hybrid sturgeon [Acipenser baerii (♀)×A. schrenckii (♂)]

Abstract

References

Catalog

Related

Export File

Citation

Format

Content