Sequence comparison of mitochondrial DNA 12S rRNA region between Eriocheir sinensis and Eriocheir japonica

The primers of Eriocheir sinensis and Eriocheir japonica were designed by using the sequences of mitochondrial DNA 12S rRNA region of Drosophila yakuba and Daphnia pulex . 457 base pairs of encoded 12S rRNA region of mitochondrial DNA were amplified and sequenced. The length of sequence of two species was the same, the A, T, G, C content s were similar, which were 159bp( 34. 79%) , 177bp( 38. 73%) , 51bp( 11. 16% ) , 70bp ( 15. 32% ) and 159bp ( 34. 79%) , 178bp ( 38. 95%) , 50bp ( 10. 94% ) , 70bp ( 15. 32% ) respectively. Between E. sinensis and E . japonica , there were 5 different sites, that were transition sites at 98bp, 151bp, 317bp, 417bp, and transversion site at 294bp.