Effects of infusing EAA pattern solution on protein synthesis in whole body, muscle and hepatopancreas of Ctenopharyngodon idellus

The experiment adopts oesophageal infusion with large dose DL242 3H 2Phe to research protein synthesis metabolism in whole body , muscle and hepatopancreas of 20 ～30g grass carp in different essential amino acid (EAA) patterns. The results show that : 1. Four imbalance EAA patterns , respectively short of Lys 、 Met 、Trp 、Arg , compared with EAA pattern of muscle , result in remarkable fractional protein synthesis rate (FSR) decrease of muscle , hepatopancreas and whole body , which shows the decreasing by Met > Lys > Arg > Trp. 2.Followingthevariationof EAApattern, FSRof muscle, hepatopancreasandwholebodyhasasimilar change , and shows FSR by : hepatopancreas > whole body > muscle. 3. In Met ,Lys ,Arg and Trp four factors of EAA pattern , from Met to Trp have gradually and orderly weakened impact on protein synthesis in muscle and hepatopancreas of grass carp , in other words , Met has the largest impact .