温度和盐度对羊鲍胚胎发育及幼虫发育的影响

Effects of temperature and salinity on embryonic and larval development of Haliotis ovina

  • 摘要: 为探究水温和盐度对羊鲍胚胎发育和幼虫发育的影响,以人工驯化后的野生羊鲍为实验材料,采用温度、盐度单一和双因子组合方法设计实验。结果表明,羊鲍孵化率和成活率受温度、盐度影响显著,28℃时,胚胎发育时间最短,孵化率最高,26℃时,幼虫发育时间最短,成活率最高。胚胎发育适宜温度为24~28℃,最适温度为26~28℃。盐度为24时,羊鲍胚胎发育和幼虫发育时间最短,孵化率和成活率最低;盐度分别为30和32时,孵化率和成活率最高。胚胎发育和幼虫发育的适宜盐度为28~32,最适盐度为30~32。温度盐度交互作用对羊鲍孵化率影响差异不显著,对成活率影响差异显著。通过响应曲面模型优化,得出温度为24.61℃,盐度为32时,孵化率和成活率均达到最大值,分别为95.32%和81.20%,满意度为0.848。研究表明,羊鲍胚胎发育和幼虫发育对温度、盐度的敏感性不同,对高温高盐的耐受性强于低温低盐。

     

    Abstract: In order to investigate the effect of temperature and salinity on Haliotis ovina embryonic and larval development in Wenchang Hainan during November to December, 2013, we used the artificial domestication of wild H. ovina as experimental material, and single or double factor combination method with the temperature and salinity to design the experiment. The results showed that: the hatching rate and the survival rate of H. ovina were significantly affected by temperature and salinity. When the temperature was 28℃, the embryonic development time was the shortest and the hatching rate was the highest. When the temperature was 26℃, the larval development time was the shortest and the survival rate was the highest. The range of suitable temperature for embryonic development was 24-28℃, and the optimal temperature was 26-28℃. The range of suitable temperature for larval development was 26-30℃, and the optimal temperature was 26-28℃. When the salinity was 24, the embryonic development time and the larval development time were the shortest but the hatching rate and the survival rate were the lowest. The hatching rate and the survival rate were the highest at 30 and 32, respectively. The range of suitable salinity for embryonic development and larval development was 28-32, and the optimal salinity was 30-32. There was no siginificant effect of temperature and salinity interation experiments on the hatching rate, but there was siginificant effect on the survival rate. Based on the response surface model, the hatching rate and the survival rate reached the maximum(95.32%, 81.20%) when the 2-factor combination was 24.61℃ and 32, with the desirability value being 0.848. The studies indicated that the H. ovina embryonic development and larval development had different sensitivity to different temperature and salinity, The high temperature and high salinity were better tolerated than the low temperature and low salinity.

     

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