Abstract:
In order to study the effects of fish oil under different stored conditions on growth performance and antioxidant indexes in juvenile grouper,
Epinephelus coioides. The conditions which the fish oil was oxidized are as follows:stored temperature:4℃, ambient temperature(AT):(31.5±3.5)℃; stored time:45, 90,135 d; supplementations of antioxidant:30 mg/Kg(EQ), 300 mg/Kg(HEQ). According to the different treatment conditions of fish oil, there were 14 groups:T
F+EQpositive control (fresh oil+ EQ), T
Fnegative control (fresh oil), T
4℃+45d+EQ, T
4℃+45d+HEQ, T
4℃+90d+EQ, T
4℃+90d+HEQ, T
4℃+135d+EQ, T
4℃+135d+HEQ, T
AT+45d+EQ, T
AT+45d+HEQ, T
AT+90d+EQ, T
AT+90d+HEQ, T
AT+135d+EQ, T
AT+135d+HEQ. Preparation of nitrogen and other fat diets fed the
Epinephelus coioides, which is from juvenile grouper (18±0.02) g to 56 days. There were no significant differences between T
F+EQ and T
4℃+45d+EQ, but T
4℃+45d+EQ was significantly higher than T
AT+135d+EQ in WGR. The FCR of T
4℃+45d+EQ, T
4℃+45d+HEQ, T
AT+45d+EQ and T
AT+45d+HEQ were relatively low, which had no significant differences with T
F+EQ and T
F but significantly lower than T
AT+135d+EQ and T
AT+135d+HEQ (
P<0.05). The cell structures of T
F+EQ, T
4℃+45d+HEQ were clear and obvious and the sizes of these fat droplets in cells were normal, but much more greater fat droplets in T
AT+135d+EQ and T
AT+135d+HEQ. The CAT of T
4℃+135d+EQ, T
AT+135d+EQ and T
AT+135d+HEQ were significantly higher than T
F+EQ and T
F. The SOD and GSH-Px were highest inT
AT+135d+EQ, which were significantly higher than T
F+EQ and T
F. The MDA of T
F+EQ, T
F, T
4℃+45d+EQ, T
4℃+45d+HEQ, T
AT+45d+EQ and T
AT+45d+HEQ in liver had no significant differences, but were significantly lower than the other groups. We found the growth performance and antioxidant index in juvenile grouper of T
4℃+45d+EQ, T
4℃+45d+HEQ, T
AT+45d+EQ and T
AT+45d+HEQ were relatively good, T
4℃+45d+EQ, T
4℃+45d+HEQ were better than T
AT+45d+EQ and T
AT+45d+HEQ. That shows short term storage, adding antioxidants are advantageous to storage of fish oil, besides 4℃ is better than ambient temperature.
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