天然水域人工模拟产卵环境诱导长江鲟自然繁殖后代的亲权分析

Parentage analysis of offspring induced by artificial simulated spawning environments in natural waters for natural breeding of theAcipenser dabryanus

  • 摘要: 2023年天然水域人工模拟产卵环境下诱导长江鲟自然繁殖试验取得成功,为了评估野化繁殖实验效果,深入了解长江鲟的繁殖交配模式,实验采用线粒体基因和24对微卫星组合标记,对亲鲟及其后代进行了亲子鉴定和遗传多样性分析。结果显示,20尾亲鱼中有7尾雄鱼和7尾雌鱼参与了繁殖。240尾子代与20尾亲鲟共享2个相同的线粒体基因单倍型。240尾子代来自30个不同的父母本组合,有223尾子代能成功匹配到其父母亲本,匹配成功率达92.92%。遗传多样性分析显示,20尾亲鲟群体的遗传多样性较高,适合作为繁殖亲本。后代群体的遗传多样性与亲鲟基本一致,表明野化繁殖实验有效地保持了后代群体的遗传多样性。3尾雄性亲鲟对后代的贡献率高达78.92%,4尾雌性亲鲟对91.48%的后代有遗传贡献。研究表明,长江鲟在繁殖过程中采取了多雌多雄的混合交配策略,这对于长江鲟自然种群的遗传多样性保护是一种有效的繁殖策略。本研究不仅为长江鲟的遗传多样性和繁殖遗传管理提供了有用信息,而且对将来实施野化放归和种群恢复工作具有重要指导意义。

     

    Abstract: In 2023, a successful natural breeding experiment for the Acipenser dabryanus was conducted in an artificial simulated spawning environments within natural waters. To assess the effectiveness of this wild breeding experiment and to gain a deeper understanding of the breeding and mating patterns of Acipenser dabryanus, this study employed the mitochondrial Dloop region and 24 microsatellite loci for parentage assignment and genetic diversity analysis of parents and their offspring. The results showed that among the 20 parental sturgeons, 7 male and 7 female individuals participated in reproduction. Mitochondrial DNA analysis indicated that 240 offspring shared two identical mitochondrial haplotypes with 20 parents. Furthermore, microsatellite loci analysis revealed that these 240 offspring originated from 30 different parental combinations, with 223 offspring successfully matched to their parental lineage, resulting in a high matching success rate of 92.92%. Genetic diversity analysis indicated that the 20 parents sturgeon exhibited a high level of genetic diversity, making them suitable as breeding stock. The genetic diversity of the offspring group was essentially consistent with that of the parents, indicating that the wild breeding experiment was effectively maintained the genetic diversity of the offspring group. Three male parents contributed up to 78.92% of the offspring, while four female parents had genetic contributions to 91.48% of the offspring. This results demonstrate that A. dabryanus adopts a mixed mating strategy involving multiple females and males. This reproductive strategy is effective for conservation genetic diversity within the natural population of A. dabryanus. This study not only provides valuable information for the genetic diversity and reproductive genetic management of A. dabryanus but also has significant guiding importance for future wild release and population recovery efforts.

     

/

返回文章
返回