Abstract: In this experiment, a basal diet containing 50% fishmeal served as the control. Spirulina was used to isoproteically replace 5%, 10%, 15%, 20%, 25%, and 30% of the fishmeal in the basal diet. Juvenile Monopterus albus with an initial body weight of (20.72±0.04) g were fed these experimental diets for 60 days. Growth performance, antioxidant capacity, immune response, hepatointestinal histology and intestinal microbiota of the juvenile M. albus were analyzed. The final weight, weight gain rate and specific growth rate of juvenile M. albus all showed a trend of increasing first and then decreasing with the increase of the proportion of Spirulina replacing fishmeal. With the highest value in the group replacing 10%, which was significantly higher than that in the group replacing 25% and 30%. The protein deposition rate showed a decreasing trend, the protein deposition rate in 25% and 30% fishmeal groups were significantly lower than those in the control and 5% groups. The feed coefficient showed an upward trend, and the feed coefficient in 25% and 30% groups were significantly higher than those in the control and 5% group. The regression analysis with weight gain rate as the evaluation index showed that the appropriate replacement ratio of Spirulina to fishmeal was 10.49%. With the increase of the proportion of fishmeal as a substitute, the activity of T-AOC and SOD in the liver of juvenile M. albus showed an increasing trend. The activity of T-AOC and SOD in the groups with 20%, 25% and 30% replacement was significantly higher than that in the control group. Except for the group with 5% replacement, the MDA content in the juvenile M. albus was significantly lower than that in the control group. With the increase of replacement ratio, the contents of LZM, ACP, AKP, IgM and C3 in the serum of juvenile M. albus showed a trend of rising first and then decreasing, which were the highest in the replacement group 15%, and significantly higher than that in the control group. No effect of Spirulinareplacing fishmeal on the liver tissue of juvenile M. albus. Both the 25% and 30% replacement groups showed significantly lower intestinal villus height, lamina propria thickness, and villus height to crypt depth ratio in Juvenile M. albus compared to the control group. At the phylum level, with the proportion of alternative fishmeal, the relative abundance of intestinal desulfurobacteria and Proteobacteria decreased, while the relative abundance of Firmicutes, Fusobacterium and Bacteroidtes increased. At the genus level, the relative abundance of Acinetobacter, Plesiomonas and Sphingomonas decreased with the proportion of alternative fishmeal, and the relative abundance of Cetobacteriumincreased with the proportion of replacement. The replacement of some fishmeal by spirulina can improve the antioxidant capacity and immunity of M. albus, improve the richness of intestinal beneficial bacteria, benefit intestinal health, and reduce the risk of juvenile M. albus. Taking the weight gain rate as the evaluation index, the suitable proportion of spirulina to replace fishmeal was 10.49%.