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第四代凡纳滨对虾抗WSSV选育家系的抗病及免疫特性研究

Studies on WSSV-resistant and immune characteristics of the 4th generation selective breeding families for resistance to the white spot syndrome virus(WSSV)of Litopenaeus vannamei

    摘要
  • 摘要: 35个第四代凡纳滨对虾抗WSSV选育家系和未选育对虾按每克体重注射103拷贝WSSV病毒量,根据选育家系的抗病性能分3个类群:高抗性类群,成活率达24.45%±6.56%;中抗性类群,成活率为10.70%±1.41%;敏感类群,成活率为2.72%±2.76%,各类群间差异显著(P＜0.01)。对分别代表高抗、中抗和敏感类群的12、7和3号家系以及未选育对虾按每克体重注射102、103、104和105拷贝 WSSV,高抗性对虾在102、103、104及105感染水平下的存活率分别为100%、23.3%±3.5%、7.8%±1.9%和0%;中抗对虾分别为87.7%±3.9%、12.2%±1.9%、0%和0%;敏感对虾分别为54.4%±3.9%、2.2%±1.9%、0%和0%;未选育对虾分别为51.1%±5.1%、0%、0%和0%。在103拷贝组感染过程中免疫相关因子的变化表明,高抗对虾血液中血细胞数分别比中抗、敏感和未选育对虾提高20.7%(P>0.05),36.7%(P<0.05)和34.4%(P<0.05);PO活力上述三类对虾提高40.0%(P<0.05),76.3%(P<0.05)和63.4%(P<0.05);SOD活力分别比上述三类对虾提高31.1%(P>0.05),58.8%(P<0.05)和32.0%(P>0.05);POD活力分别比上述三类对虾提高29.6%(P>0.05),44.9%(P<0.05)和43.3%(P<0.05);血清蛋白含量分别比分别比上述三类对虾提高31.2%(P>0.05),38.7%(P<0.05)和39.3%(P<0.05),而敏感对虾和未选育对虾之间则无显著差异。结果表明,经四代选育后的高抗对虾免疫性能明显高于其他对虾,表现出良好的抗WSSV性能。

     

    Abstract: In this paper,in comparison with their WSSV-resistant characteristics,35 selective breeding families of the 4th generation and no selected shrimp of Litopenaeus vannamei were divided into three groups:relatively high resistant families,mid resistant families and susceptible families,with the average survival rates of 24.45%±6.56%,10.70%±1.41% and 2.72%±2.76%,respectively after 103 copies WSSV/g(body weight)infection,which were significantly different among the three groups(P<0.01).Injected WSSV of 102,103,104 and 105 copies WSSV/g,the survival rate of the relative high resistant family 12 was 100%,23.3%±3.5%,7.8%±1.9% and 0 %,respectively; and the survival rate of the mid resistant family 7 was 87.7%±3.8%,12.2%±1.9%,0% and 0%,respectively; and the survival rate of the susceptible family 4 was 54.4%±3.8%,2.2%±1.9%,0% and 0%,respectively; and the survival rate of non-selected was 51.1%±5.1%,0%,0% and 0%,respectively.In comparison with mid-resistant family 7,susceptible family 3 and non-selected shrimp,the THC in relatively high resistant family 12 increased by 20.7%(P>0.05),36.7%(P<0.05)and 34.4%(P<0.05)compared with that of the above-mentioned shrimps,respectively after 103 copies WSSV/g(body weight)infection; the PO activities increased by 40.0%(P<0.05),76.3%(P<0.05)and 63.4%(P<0.05)compared with that of the above-mentioned shrimps,respectively; the SOD activities compared with 31.1%(P>0.05),58.8%(P<0.05)and 32.0%(P>0.05)increased by that of the above-mentioned shrimps,respectively; the POD activities compared with 29.64%(P>0.05),44.9%(P<0.05)and 43.3%(P<0.05)increased by that of the above-mentioned shrimp s,respectively; the content of plasma protein compared with 31.2%(P>0.05),38.7%(P<0.05)and 39.3%(P<0.05)increased by that of the above-mentioned shrimps,respectively.However,there were no significant differences of the THC,PO,SOD,POD and content of hemolymph protein among mid-resistant family,susceptible family and non-selected shrimp.After 4 generations of selection,the immune capability of the shrimp from relatively high resistant family was much higher than that of the other groups and non-selected shrimp,with apparent powerful capability of WSSV resistance.These findings would help to direct the future selective breeding of shrimp for WSSV resistance.

     

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