Abstract: Half-smooth tongue sole(Cynoglossus semilaevis G?nther)is distributed along the coast to the Yellow Sea,and Bohai Sea.It is a cultured marine fish species which is highly valued because of its delicious,tender meat and nutrition.However,diseases of the farmed fish have taken place constantly and yield losses due to epidemic diseases restrict earnings and progress of fishery.To develop new strain with enhanced disease-resistance and fast growth is an important task in tongue sole aquaculture.In the present study,wild population from Bohai sea and cultured population were used as basic populations.18 families of half smooth tongue sole were established.Growth comparison indicated that significant differences were observed in growth rate among the 18 families.Among these 2 fast-growth families(family 15 and 16),4 secondly fast-growth families(family 6,7,16 and 27),and 12 common growth families(family 1,3,4,10,12,14,19,24,27,28,33 and 34)were obtained.12 families were chosed for pathogenic bacteria, Vibrio anguillarum,challenge experiments which showed that one family(family 2)had strong disease resistance with the survival rate of 79%;2 families(family 12 and 14)had secondly strong disease resistance with the survival rate of 50%-60%;6 families(family 3,6,7,10,16 and 19)had common disease resistance with the survival rate of 35%-50%;and 3 families(family 15,27,and 30)had low disease resistance with the survival rate of less than 35%.