Abstract: The Duliu River is located in the Southeast Guizhou,China and is one of the great rivers in Guizhou.It belongs to the Pearl River system and is situated at 107°34′-109°35′E and 25° 19′-26° 29′ N.The length of the Duliu River is 365 km and the basin area of the river is about 15 000 km².The river runs swiftly in deep valley and is full of shallow beaches.The Duliu River is located in the mid-subtropical zone with humid monsoon climate and has high biodiversity.To supply scientific data for the protection and utilization of the fish resources in the Duliu River,the fish fauna and zoogeological division of the river were studied according to my field survey of fishes for the river from 2002 to 2010,integrating with related reference.The results show that a total of 88 species(subspecies),belonging to 4 orders,13 families and 56 genera,were distributed in the Duliu River.Of them,28 species were found for the first time to be distributed in the Duliu River,6 species comprising Schistura fasciolata,Discogobio brachyphysallidos,D.yunnanensis,Abbottina rivularis,Pseudobagrus adiposalis and Hypseleotris swinhonis were first found to live in the Liujiang River system,and 3 species including Discocheilus multilepis, Beaufortia intermedia and Siniperca fortis were endemic to the Duliu River.The fishes in Cyprinidae,Cypriniformes were dominant in the fish fauna of the Duliu River and account for 75.0% of fishes in the river.The fish fauna in the Duliu River is characterized by high species diversity,abundant species endemic to the Pearl River system,lack of the species in the Subfamily Schizothoracinae and the Genus Triplophysa,and no migratory fishes and Karst cave fishes.The fishes in the Duliu River originated in the early Tertiary and belong to 4 fauna complexes respectively.The fish fauna in the Duliu River belongs to the South China Division in the Southeast Asiatic Subregion of the Oriental Region.