Abstract: The gamete quality of F₁-generation Chinese sturgeon(Acipenser sinensis)was evaluated by a series of biological indexes of eggs(diameter,weight,fertilization rate and hatching rate)and sperm(the highest activation rate,life time and fast movement time).And the growth characteristics of F₂-generation(2012-11-2013-07)were analyzed by following up the development situation.Results showed that the eggs of F₁-generation were full shape and the average diameter was 3.71 mm,which was less than that of wild population.The fertilization rate(60.1%)and hatching rate(36.8%)were both at lower-middle level when compared with data over the years.Sperm motility in 2012 and 2013 showed that the effective moving time(circinate movement time+fast movement time)were longer than those of wild population.It indicated that eggs quality of F₁-generation was lower than that of wild population,and sperm quality was higher yet.In comparison with F₁-generation cultured by intensive farming system in the same aquaculture base over the years,F₂-generation showed obvious growth phase differences.That is,the growth rate was slower in larvae phase but faster obviously in juvenile and young fish phase.Consequently,it was effective to get high quality gamete and F₂-generation by improving the gonad development perfection of cultured F₁-generation in order to prevent the degradation of germplasm.And it was vitally important for Chinese sturgeon species conservation.