毛蚶维生素B12的提炼及其吸收光谱的检定

JHE EXTRACTION OF VITAMIN B12 FROM ARCA SUBCRENATA AND THE DETERMINATION OF ITS ABSORPTION SPECTRA

  • 摘要: 1.本文研究了毛蚶维生素B12的含量及其提炼方法。试验证明:鲜毛蚶肉每百克含B1265~85微克,在加工生产中蒸煮的蚶汤合B120.20~0.30微克/毫升,蚶肉浸出液含B120.25~0.40微克/毫升。 2. 从毛蚶中提取的B12为暗红色针状结晶,其三个特征吸收峰分别位于278.3毫微米、361.0毫微米和550.0毫微米,峰值光密度之比为: A361/A278=1.72, A361/A550=3.25 表明毛蚶B12结晶特性符合《中华人民共和国药典》的规定(1963年)。

     

    Abstract: 1. The vitamin (12) content of the Mollusk (Arca subcrenata) and its extr-action methods have been studied. The results obtained indicate that the vitamin B(12) content of the fresh meat of Arca subcrenata is 65~85r/100g., of its brothobtained during processing, 0.20~0.30r/ml. and of the extractive of its meat0. 25~0. 40r/ml. 2. The vitamin B(12) extracted from Arca subcrenata consists of needle-shapercrystals of dark red color. Its three absorption peaks are found at 278.3mμ,361.0 mμ, and 550.0 mμ. The ratios of absorbency are: A361/A278=1.72, A361/A550=3.25 It was confirmed that the characteristics of vitamin B(12) crystals extractedfrom Arca subcrenata well agree with the descriptions in Chinese PharmaceuticalCode (1963).

     

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