Abstract: The process of nuclesr-insertion in pearl oyster includes three stagers: preparation, inserting operation and recuperation. During the preparation stage, the function of molluscs decreases gradually, the action of addueter-shell movement becomes weak and less frequent, the time of shell elossing is extended. The average frequency and the a-erage amplitude of shell opening is about half of the regular ones and shell closing period estends shout 1 to 2 times. These changes are objiously observed. After each experiment mast of pearl oysters are alive in reUperation, sametim the survival rate reaches to lUU.At the same time, izz inserting operation most of the pearl oyster can open their shells by their own, which brings gxeat cane enience to operatiVn. Tn recent years average survival rate reaches to 91,and the death rate in recuperation (one month) reduced to Iower than 15.his erperiment has basically solaced the key terhnical problem of high death rate in recuperation stage.