草鱼肾组织细胞系CIK的建立及其生物学特性

A CELL LTNE DERTVED FROM THE KIDNEY OF GRASS CARP CTENDPHAIYNCDDDIV IDELLUS

摘要: 作者于1982年1月开始进行草鱼肾组织单层细胞培养,至今已连续培养32个月,传至l20多代,建立了细胞系,定名为草鱼肾组织细胞系(CIK)。本文介绍了原代和传代培养、细胞形态、细胞生长速度和分裂指数、细胞的保存和对温度的适应性、细胞染色体分析以及细胞系对病毒敏感性的试验和研究结果。CIK生长迅速、适应性强、以20°—38℃生长较好,28℃左右生长稳定,pH为6.5时仍能保持致密单层,染色体数为非整倍体,众数为55。对草鱼出血病左右病毒FRV具敏感性。

Abstract: A fibroblast-Like cell line derived from kidney of healthy Grass Carp (Otenoaolaayragodon 2deLtus) was esablisbed and designated CIK which has been passed for126 time since its initiation in January, 1982. The medium used in the cultivation is Eagle MEM (Nissui eiyaku Co LTD)supplemented with 10-15% fetal bovine serum. The incubation temperature is at 28℃. Chromosome number at passage level of 88 ranked from 28 to 117 with a rxiodalnumber of 55.CIK cell line is susceptible to FRV.