Abstract: A few antisera against fish reovirus(FRV) were prepared by our labor-story. An enzyme-linked immunosorhent assay (ELISA) and discounter imnaunoele-ctropharesis (DCTE) were developed to do the serological. diagnosis of FRV infection in grass carp, using these antisera. The DCIE was compared with ELSA for deteo-tion of FRV. The EL1SA was found to be at least 400 times mare sensitive than the DOIE，and as a highly sensitive, rapid and specific method for early diagnosis of FRV.