Abstract: In this paper, physiogical characteristics and drug sensitivity of vibriocholera (non-01)isolated from the ulcerous eyeballs and hemolyph of disoased penacidshrimp(Penaeus orientalis Kishinouye) are desribcd. Effects of temperature, salinityand pH on the growth of the organism were examined: It grew in 1% peptone waterat temperatures of 20-45℃ (Optimum range 30-42℃), at salinity of 0-5% (Optimum0.5-2%) and a pH from 5 to 10 (Optrium6-9) rspectively. All the isolated strains were highly sensitive to Terramycin, Chloramphenicol,.Tetracycline, Erythromycin, Gentamycin,Kanamycin and Neomycin and also to Co-trimoxazole, Furazolidone and Nitro-furantoin. But they were not sensitive to Penici-llin G, Vancomycin and Bacitracin. In the solution of bleaching powder (contains active chlorine 30%) at 0.2 ppmfor 60 minutes, the bacteria were 95.5% killed. It was successfully treated with blea-ching powder st 1 ppm in the oulture water for controlling this vibrio cholera (non--01)in penaeid shrimp.