葡萄球菌A蛋白协同凝集试验快速检测草鱼出血病病毒

RAPID SEROLOGICAL DIAGNOSIS OF GRASS CARP HAEMORRHAGIC VIRUS BY COAGGLUTINATION TEST USING ANTIBODY-SENSITIZED STAPHYLOCOCCI PROTEIN A

  • 摘要: 本文报道葡萄球菌 A蛋白协同凝集(SPA COA)快速检测草鱼出血病病毒的方法以及对提纯病毒、染毒细胞内病毒和病鱼组织检测的初步结果。并对该方法进行了特异性和敏感性考核以及凝集物的电镜观察。该方法快速、特异、设备简单,适合基层单位检测草鱼出血病病毒,并有可能作为草鱼出血病疫苗质量鉴定的指标之一。

     

    Abstract: This study deals with the rapid diagnosis of grass carp (Ctenopharyngodon idellus) haemorrhagic virus by coagglutination test using antibody-sensi-tized staphylococci protein A (SPA COA), as well as the preliminary experimentaldata for the diagnosis of purified vius, cell-infected virus and tissues of infectedfish. The results obtained from the specific and sensitive detection and the electro-nic microscopic observation of the coagula, indicate that this rapid and simple me-thod, which requires no special apparatus and easy to perform in the basic unit, isprobably one of the valuable indexes for identifying the vaccine quality of grasscarp haemorrhage.

     

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