条斑紫菜细胞苗培养及总氮量、氨基酸分析

CELL SEEDLING CULTURE AND TOTAL NITROGEN AND AMINO ACID ANALYSIS IN PORPHYRA YEZOENSIS

  • 摘要: 我们用条斑紫菜体细胞进行室内育苗,并下海养殖。对细胞苗养成的紫菜的总氮量及氨基酸量进行了测定,并与传统法育苗养成的紫菜的总氮量及氨基酸量进行比较。结果表明,细胞苗紫菜与传统苗紫菜的总氮量及总氨基酸量相近,说明从群体上看,两者的主要营养成份无甚差异,营养价值相近。

     

    Abstract: Seedlings from the culture of somatic cells of Porphyra yezoensis havebeen done in Hai Feng sea farm of JiangSu province. The isolated single somaticcells was cultured with enriched seawater in temperature-controled room at 20℃ andunder 2000 lux illumination for 12-hour daily. After few days, some cells dividedand formed seedlihgs and most cells firstly divided into cell aggregates, then theaggregates released spores that germinated into sporelings. The cell seedlings grew 0.1-1mm in length in 15-20 days. Cultured at room for 15-87 days, the cell seed-lings on nets were moved into sea and cultivated with 2 batches. After 25 days laterthe cell seedlings of 1 st batch grew to about 10-15 cm in length. The content of total nitrogen and amino acids of the thalli were analyzed fromcell seedlings and traditional sporelings, and took to compare. The results showedthere were no change in total nitrogen and toll amino acids between cell seedlingsand traditional sporelings, though there were some changes in the content of somesmino acids, so there are no differece in nutrient value between the population ofthe cell seedlings and the traditional sporelings of Porphyra yezoensis.

     

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