Abstract: The present paper describes the characters of pathogenic bacteria of Edwardsielliasis in eels in Zhejiang Province. The four strains isolated from the liver, kidney and blood of the diseased eels were shown to be the cause of the disease by infection experiment. The symptom was similar to that of natural incidence.These bacterial isolates are short rod shaped, 0.5-0.8X0.8-1.5um, arranged singly, Gram-negative, motile by peritrichous flagella, no capsule and spore, and facultatively anaerobic. Produce acid and gas from glucose, produce hydrogen sulfide and indole. Nitrate is reduced. They can utilize cellobiose, sucrose, maltose, arabinose and lactose,and can not utilize mannitol and trehalose. Citrate and malonate are not utilized as sole source of carbon, M. R. test is positive and V. P. reaction is negative. Catalase, lysine decarboxylase and ornithine decarboxyiaso are positive and oxidase,urease,lipase,arginine dehydrolase and phenylalanine deaminase are negative. They can grow in 0—3.5% NaCl, or at pH among 4.7—9.0, but can not grow in medium containing NaCl more than 3.5%, or at pH 4 and pH 10, or at 5℃ and 42℃.According to the morpholical features, physiological and biochemical characteristics of the strains E895205, they are different from those of Eduardsiella tarda, E. hoshinae, E. ictaluri and E. fujianensis. So, E895205 can be considered as a new species, and it is named Edwardsiella Zhejiangensis sp. nov. Type culture is deposited in the Zhejiang Fisheries College, Ningbo Branch, Ningbo Municipality,