中国对虾淋巴组织培养中的病毒及病理观察

VIRAL AND PATHOLOGICAL OBSERVATION IN CULTURED LYMPHOID TISSUES OF SHRIMP PENAEUS CHINENSIS

  • 摘要: 通过对中国对虾的淋巴器官和经过培养的养殖,海捕中国对虾的淋巴组织细胞进行电镜观察,均发现在细胞内存在一种形状为球形,有囊膜,平均直径为136nm的病毒。病毒分布于细胞质内,或成团存在,似为一种虹彩病毒;在养殖对虾淋巴器官及培养过的组织细胞中还发现另一种病毒,病毒粒子为正二十面体,无囊膜,平均直径为33nm,分布于细胞质内,似为一种小RNA病毒。

     

    Abstract: The lymphatic organ of shrimp Penaeus chinensis and the cultured lymphatic tissues fromfarmed and wild shrimps were examined under the transmission electronic microscope (TEM). The particles with envelope , which are icosahedron in shape and 136 nm in diameter , were found in the cytoplasm in all samples .It seemed to be the Iridovirus .Another virus was found in the cytoplasm of lymphatic tissue of farmed shrimp .The particles were icosahedron , 33 nm in diameter and without envelope , which seemed to be the Picornavirus .No significant replication of the viruses was found in the cultured cells .Pathological changes , including karyorrhexis , nuclear hypertrophy , cytoplasm vacuolization , tissues necrosis of lymphatic organ were also observed .

     

/

返回文章
返回