Abstract: Through several years.. research, we successfully obtained the progenies of the distant hybridization between Oreochromis aurea( .. ) and Sinipperca chuatai ( .. ) . Traditional morphological data and truss network data were combined to conduct multivariation analyses to study the morpho logical variations among O. aurea f rom American(Ao) , progenies of Ao( .. ) .. S. chuatai ( .. ) ( As1) , O. aurea from Egypt ( Eo ) , progenies of Eo( .. ) .. S . chuatai ( .. ) ( Es1) , as well as so..called back..crossing of the As1 ( As2) , and sister..brother crossing of Es1( Es2) . Ch..square analysis of merist ic characters indicated that there were no signif icant differences among these six experimental f ishes. But the results of cluster analysis of metric characters plus truss network data showed that there were some differences between these six experimental f ishes and especially, As1 and Es1 were quite different from the other fishes, though no difference was detected between As2 and Eo . Discriminant analysis indicated that there were significant differences among these six fishes. The identif icat ion accuracy was ranked as As1, Es1 and As2 100% > Es2 96. 2% > O. aurea A 95% > O. aurea E 90% . According to the results of mult ivariat ion analyses and subspecies 75% rule, the progenies of O. aurea .. S. chuatai are different from O. aurea. So it is reasonable to improve aquaculture strains using distant hybridization.