黄芪对鲤头肾中巨噬细胞的增殖和一氧化氮产量的影响：离体研究
Effect of Astragalus radix on proliferation and nitric oxide production of head kidney macrophages in Cyprinus carpio :an in vitro study
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摘要: 用密度梯度离心分离鲤头肾中的巨噬细胞和嗜中性粒细胞.用RPMI细胞培养液对分离的细胞进行原代培养,在细胞培养液中加入不同浓度的黄芪水提取液来研究黄芪对鲤头肾中免疫细胞非特异性免疫应答的影响.黄芪水提取液单独使用时能刺激鲤头肾中巨噬细胞的增殖,但不能刺激巨噬细胞的氮暴发活性.用黄芪和脂多糖(LPS)混合刺激巨噬细胞和中性粒细胞时,黄芪水提取液能显著提高脂多糖刺激所产生的一氧化氮的产量.研究结果表明,黄芪不仅能刺激巨噬细胞数量的增加,而且能协同脂多糖增强头肾中免疫细胞的功能,从而对机体的非特异性免疫起调节作用.Abstract: The in vitro effect of Astragalus radix water extract on the non2specific immune responses of macrophages isolated from the head kidney of Cyprinus carpio was evaluated. Astragalus radix extract stimulated the proliferation of the head kidney macrophages , and it alone had no effect on nitrogen burst activity of macrophages. But it enhanced the nitric oxide production of the mixture of macrophages and granulocytes induced by LPS. It is suggested that Astragalus radix could modulate the non2specific immune response of Cyprinus carpio.
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