Abstract: The oogenesis and ovarian development cycle of Sepiella maindroni, artificial breeding, are observed on histological and cytological characteristic by using routine histological techniques. According to the size of oocytes, the morphology of nucleus and the relationship between oocytes and follicle cells, the oogenesis can be divided into four stages as follows: oogonium stage, oocytes stage, maturation stage and resorbing oocytes stage. Moreover, the histological characteristic and variational disciplinarian of oocytes at each stage is described in detail. Basis of the observation of oogenesis, the results illuminate that this cuttfish’s oogenesis are provided with particularity. About twelve days after hatching, there are oogonium in the ovary, and then the oogonium proliferate quickly. Examined with a light microscope, the oocytes is follicular type, and their development are not at the same developmental stage in the ovary. According to the oogenesis, the eggs have no primary membrane, there are only secondary membrane and tertiary membrane.The secondary membrane is excreted by follicle cells; and the tertiary membrane is made by the secretion of albumen gland、nidamental gland and ink sac. Basis of the morphological characteristic and variational disciplinarian through histological observation, the process of ovarial development of breeding Sepiella maindroni can be divided into 6 stages.