Cu2+对中间球海胆生长、免疫及性腺发育的影响

Effects of Cu2+ on growth, immunity and gonad development of Strongylocentrotus intermedius

  • 摘要: 为探讨铜离子(Cu2+)对中间球海胆生长和性腺发育的影响及调控机制,在室内水槽(200 L)中进行为期60 d的浸泡实验。实验设置测试组(0.02 mg/L Cu2+)和对照组(自然海水),每个处理设置3个重复,每个水槽养殖20只海胆(13.58±0.79) g。实验中期和末期分别测定海胆增重率(WGR)、性腺指数(GI)、抗氧化酶活性和主要卵黄蛋白基因(MYP)表达量。结果显示,30 d时,测试组的WGR和GI与对照组差异不显著,而60 d时测试组的WGR和GI显著低于对照组;60 d时,测试组海胆体腔液中过氧化氢酶(CAT)和谷胱甘肽巯基转移酶(GST)活性均显著低于30 d时的对应值,而丙二醛(MDA)含量较30 d时有所升高;60 d时,测试组抗氧化能力总体高于对照组,其中体腔液中CAT活性显著高于对照组。2次取样中,测试组性腺中MYP基因的表达量均显著低于对照组,而测试组消化道中MYP基因的表达量却显著高于对照组。60 d时,测试组海胆体腔细胞中总蛋白含量显著高于对照组,而体腔液上清液中总蛋白含量在测试组和对照组之间差异不显著。研究表明,铜离子能够引起氧化应激,降低海胆的增重率和性腺指数,这可能是通过抑制性腺MYP表达量及阻碍MYP从消化道向性腺正常转运所致。

     

    Abstract: This study was conducted to investigate the effects of Cu2+ on the weight gain rate (WGR), gonadosamatic index (GI), antioxidant enzyme activities and transcription of major yolk protein gene (MYP) in Strongylocentrotus intermedius. All experimental sea urchins were randomly divided into six tanks (200 L), and each tank was stocked with 20 individuals. Then, the test group was added CuSO4·5H2O to a final concentration of 0.02 mg/L Cu2+ with natural sea water as the control group. The experiment lasted for 60 days. Results showed that WGR and GI were significantly lower in the test group than those in the control group at 60 days. The activities of CAT and GST in the coelomic fluid of sea urchins in the test group were significantly lower at day 60 than those at day 30, and the activity of CAT was lower in the test group than that in the control group. The MYP transcription was significantly lower in the gonads of the test group than that in the control group, but the expression ofMYP was significantly higher in the digestive tract of the test group than that in the control group. The total protein content was significantly higher in the coelomocytes of the test group than that in the control group. Results above showed that the WGR, GI and antioxidant enzyme activities of sea urchins could be reduced by Cu2+. This may be caused by inhibiting MYP expression in the gonad and the MYP transportation from the digestive tract to the gonad.

     

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